The main character of the series, age fourteen and hailing from the Fuso Empire. A typical young girl who comes from a family of clinicians who use their magic powers to heal people. Yoshika gains the features of a mame-shiba when using said power. Her father designed the Striker Units the Strike Witches use to fight. However, because her father died in the war she is vehemently against fighting when Mio Sakamoto appears to recruit her. Only after entering combat for the first time, helping to defend the Fuso fleet heading to Britannia from a Neuroi attack, does she decide to join the 501st. Now she holds the rank of Sergeant. Yoshika has a talent for cooking, but tends to care more about her food's health benefits than its taste, such as when she introduced the other Witches to natto. Carries a 13mm Type99 Machine gun along with a M712 in battle. She has a small gray dog as a familiar which does not appear in the anime series. Her Striker Unit is modeled after the A6M Zero and she is based on the ace Kaneyoshi Mutoh.
The first Strike Witch and sub-commander of the unit, with the commissioned rank of Squadron Leader and official rank of Major. Mio is also the oldest witch at twenty years old and, like Yoshika, comes from Fuso. She is seemingly the most tough and mature of the Witches, being an experienced veteran of the early fighting against the Neuroi. She knew Yoshika's father while he was first designing the Striker Unit and was the first person to pilot one. Her usual task is to train new recruits, and in this role she becomes a very strict disciplinarian, appropriately gaining the ears and tail of a Doberman when equipped with her Striker. Otherwise she is quite friendly, if a little too forward at times, with a very distinct laugh. Under Mio's eyepatch is a magic eye that grants her increased visual prowess and the ability to spot Neuroi cores when used. Being 20 years old however Mio's magical power has aged too far for her to erect effective shields in combat, however she can still fly and use her magical eye. Carries a 13mm Type99 Machine-gun into a battle along with a magical katana strapped to her back capable of cutting apart giant Neuroi. Her Striker Unit is based on the A6M Zero and she herself is based on Saburo Sakai and Tetsuzō Iwamoto.
The ranking officer of the 501st Joint Fighter Wing at eighteen years old, Minna has a sisterly air that shows her concern for those under her command. Whether in or out of battle, her first priority is the safety of her subordinates. Her more gentle demeanor means that she often acts as the "good cop" to Mio's "bad cop" when it comes to discipline. She also has a talent for singing, wanting to become a professional before she joined the war effort. However, she gave up that dream when her lover was killed in battle. Because of this, she is afraid of losing anybody else, which leads her to eventually give an order that prohibits the male workers on the base from having unauthorized contact with the witches. Alluding to her namesake, Minna gains wolf features when equipped for combat. She has the power of clairvoyance used to analyze situations such as enemy muster, hidden spies and identify targets. Holds the commissioned rank of Wing commander, official rank of Lieutenant Colonel, and her affiliation is the Karlsland JG 3 Luftwaffe. During combat she arms herself with an MG42. Her Striker Unit model is the Messerschmitt Bf-109G-2 and her ace pilot archetype is Wolf-Dietrich Wilcke.
Fifteen years old, Lynette is the only Witch in the 501st who is native to the Britannia Federation. The middle child of eight siblings, Lynette developed very domestic and practical habits from helping to take care of her them. Similarly domestic is the Scottish Fold, from which her Striker-equipped animal features are based. Plagued by occasional clumsiness and sometimes overlooked, she nevertheless strives to do her best. Holds the rank of Sergeant and her affiliation is the Royal Air Force 610th Fighter Squadron. Her armaments are a Mk. I Boys Anti-tank Rifle and a Bren light machine gun. She serves as the squadron's sharpshooter and possesses an almost magical degree of eyesight similar to Mio's. Her Striker Unit is based on the Supermarine Spitfire Mk IX, and her pilot archetype is Billy Bishop.
A wealthy and proper fifteen year old Gallian girl who looks down on some of the less-refined habits of her peers. The emotional scars left by the Neuroi's destruction of her homeland cause her numerous problems. She often refuses to work as part of a team, and develops a dislike for the upstart newcomer Yoshika due to her attachment to Mio, which eventually develops into full-blown jealousy, even leading her to challenge Yoshika to a duel (as seen in episode 9). When wearing her Striker Unit, she gains the dark ears and tail of a Chartreux. Perrine holds the rank of Flying Officer and her original unit was the Armée de l'Air 802nd Flying Corps. Depicted carrying a rapier in artwork which does not make an appearance and uses a Bren light machine-gun. She has a lightning based spell called Tonnerre which can strike down multiple targets. Her Striker Unit is based on the Arsenal VG 39 and she is named after Pierre Clostermann.
Karlsland's sixteen year old "ultra ace" of the Luftwaffe JG 52 with more than 200 confirmed victories and the rank of Flying Officer. While extremely efficient and strategic in battle, Erica is surprisingly sloppy with her belongings and often neglects cleaning tasks. The type of person who is only serious when the situation calls for it. Her special technique is an offensive barrel roll named Sturm. With her Striker Unit equipped, Erica gains Dachshund features. Her armaments are the MG42 and a MP40. Her Striker Unit is based on the Messerschmitt Bf-109G-6 and her ace archetype is Erich Hartmann.
Eighteen year old Witch from Karlsland; a Flight Lieutenant formerly of the Luftwaffe JG 52. Quiet, efficient, and private, Gertrud (known as "Trude" to her friends) tends to support her comrades from the shadows. Her naturally calm attitude grants her great battlefield stamina, allowing her to rack up an impressive shot-down tally. A long-time acquaintance of Minna and Erica, she is often seen supporting the former in her leadership tasks and chastising the latter for her sloppiness. She has a younger sister named Chris who was badly injured during the invasion of her country and vaguely resembles Yoshika. Her magical power grants her the most physical strength of all the Witches to the extent where she can lift a steel girder many times her size. In flight she gains the features of a German Wirehaired Pointer. For battle her armament consists of an MG42, MG131, or MG151 which she dual wields. Her Striker Unit is based on the Focke-Wulf Fw 190D-6 and her pilot archetype is Gerhard Barkhorn.
Joining the 501st from the Duchy of Romagna, Francesca is the youngest soldier of the unit at a mere twelve years. Her rank is that of a Warrant officer and her unit affiliation was with Romagna's Regia Aeronautica 4th Air Unit. Francesca is often childish and self-indulgent, shirking training and other duties in order to have fun around the base or nap. However, in battle she displays a true talent and passion for flying. Also displayed are the ears and tail of a black leopard. She is good friends with the similarly casual Charlotte. She has a tendency to occasionally speak in a sing-song tone. During battles she carries a M1919 A6, Breda-SAFAT 12.7 mm machine gun, or Beretta Model 1938A. She has the power to briefly increase her acceleration which she uses to acrobatically dodge shots. Working with Charlotte, who throws her, she can also erect a lance shaped set of shields to ram enemies. Her Striker Unit is based on the G.55 Centauro, and her ace archetype is Franco Lucchini.
A sixteen year old girl hailing from the United States of Liberion with the rank of Flight Lieutenant. Magnanimous in character, tall and buxom in appearance, Charlotte ("Shirley" to her friends) is a thrill-seeker who is fascinated by speed. Like a speedy hare, she gains the ears and tail of a rabbit when equipped with her Striker (possibly alluding to model and photographer Bunny Yeager). Concerned with cleanliness, Charlotte had her personal bath transported all the way to the Strike Witches' Britannia base when she was transferred from the Liberion Army 363rd Fighter Wing. In combat her armament can consist of a M1918 Browning Automatic Rifle, Thompson M1 A1, or Colt Government M1911 A1; also depicted carrying a Bowie knife. The P-51D Mustang serves as the basis for her Striker Unit that she nicknames Merlin, while she herself is based on Chuck Yeager. Like her namesake, Charlotte becomes the first person to break the sound barrier and is the fastest Strike Witch. Before joining the 501st she was also a motorcycle racer who broke the land speed record on Bonneville Salt Flats using a modified Indian motorcycle identical to one used by legendary New Zealand speed bike racer Burt Munro in the 1950s.
The fifteen year old top ace of the Suomus Air Force 24th Unit with the rank of Flying Officer. Eila's hobby is fortune-telling with tarot cards, although the accuracy of her predictions is questionable at best. Somewhat mischievous, like the black fox whose features she gains in flight. She has a talent for analyzing the thoughts and feelings of her companions, and this skill translates well to the battlefield where her judgment is used to consistently outwit her opponents. In combat her weapons of choice are the MG42 and Suomi M1931 submachine gun. Eila's Striker Unit is based on the Messerschmitt Bf-109G-2 and she herself is based on Eino Ilmari Juutilainen.
The single Witch out of the Orussia Empire at thirteen years old. Preferring to stay indoors during the day, Sanya has been described as "nocturnal." At times she is literally asleep on her feet, counting on Eila to move her around. Despite this somewhat limiting her contact with the other members of the 501st, her low profile makes her ideal for night missions and sneak attacks. Also helping her in this duty is her magical specialty, the ability to read signals such as radio waves that are invisible to the naked eye, giving her a highly advanced spatial awareness. Gains the ears and tail of a black cat with her Striker Unit equipped. Sanya's rank is Flying Officer and her original unit affiliation is the Orussia Imperial Army 586th Fighter Regiment. Her weapon of choice is a modified Fliegerfaust called the "Fliegerhummer." Her Striker Unit design is based on the MiG60 (a fictional warbird combining features of the MiG-1, MiG-3, and Yakovlev Yak-1) and her pilot archetype is Lydia Litvyak.
The main character. An ace dogfighter from the Fuso Empire, she rose to fame after the initial Neuroi probing attack in the Fuso sea in 1937, where she shot down 7 enemy fighters. She was expecting to get deployed to the frontlines in Karlsland, but was ordered to Suomus instead. Needless to say, she's not happy about that.
Also from the Fuso Empire, stationed in a naval air squadron. She joins Tomoko in order to support her through her depression despite her reluctance to fight. However during her time there she eventually falls in a love triangle with Sumous witch Mika Ahonen of another Squadron and Tomoko...
The number 1 trouble-making insubordinate member of the Britannian Air Force. Considered to be a loose cannon compared to everyone else, Elizabeth was to be executed for her constant insubordinations and nature by the Britannian military brass. She is considered to have a rather gloomy personality with a slightly suicidal streak and often have a bit of a depressed side to her but shows supports Tomoko on her decisions. Wields a Kukri In battle. Based on George Beurling.
Hailing from Texas in the United States of Liberion, she can be a real airheaded dunce. She was transfered over after the Liberion Navy thought she could use some experience over at Sumous rather then dragging her forces down due to her rookie tactics. Despite her inexperience she helps to do her best to keep her team in high spirits.
From Karlsland, she is the younger sister of Erica Hartmann from the anime. The youngest in the squadron at 10 years of age, all she does is read. And tries to learn everything according to Karlsland doctrines.
She is nervous, timid and inexperienced, but tries her best. A member native to Suomus, she's probably one of the more "normal" ones in the bunch.
She is a member from the Romagna Dukedom.
These characters appear in the first manga (although Amaki Suwa also appears in the final anime episode.
Appeared in episode 12 of the anime. Like Yoshika, she is a Witch from Fuso. She gives Yoshika a letter from her father Dr. Miyafuji, but whether or not this means he is alive is unclear.
These characters appear in the second manga.