Aircraft

• Supermarine Spitfire, a British single-seat fighter aircraft used in World War II

Airports

• Spitfire Aerodrome, an airport in Pedricktown, New Jersey, (United States)

Automobiles

• Triumph Spitfire, a small two-seat British sports car from Triumph Motor Company developed in the 1960s

Ships

• HMS Spitfire, the name of three British naval ships
• USS Spitfire, the name of four U.S. naval ships
• UPS Spitfire, the name of two Polish naval ships

Movies

• Spitfire (1934 film), a U.S. film starring Katharine Hepburn
• Spitfire, the U.S. title of the 1942 British film The First of the Few
• Spitfire (1994 film), a U.S. film starring Lance Henriksen

Music

• Spitfire (song), a 2004 single by the British electronica act The Prodigy
• Spitfire (Jefferson Starship album), a 1976 album by Jefferson Starship
• Spitfire (UK band), a garage rock band from Crawley, West Sussex, England
• Spitfire (US band), a metalcore band from Virginia Beach, Virginia, USA
• Spitfire (Russian band), a ska band from Saint Petersburg, Russia
• The Mexican Spitfires Sydney-based Australian indie-pop band of the 1980s; sometimes abbreviated as "the Spitfires" or "the Spitties"
• Spitfire Records, a heavy metal-oriented record label

Comics

• Spitfire (comics), a fictional character in Marvel Comics World War Two-era superteam "The Invaders"
• Spitfire (New Universe), a.k.a. Spitfire and the Troubleshooters and Codename: Spitfire, a comic book published under the New Universe imprint of Marvel Comics
• Project Spitfire, a fictional US government project appearing in the Marvel Comics' series newuniversal.
• SpitFire, a character from the anime/manga Air Gear.

Other uses

• Lupe Vélez, an actress known as "the Mexican spitfire"
• Spitfire (beer), a UK bottled and cask beer
• Spitfire (BBS), a software program for operating a Bulletin Board System
• Kent Spitfires, the name adopted by Kent County Cricket Club when playing ODI or Twenty20 matches
• The Spitfires is the nickname of Eastleigh F.C., a non-league football club in Hampshire, England
• Spitfire sawfly, a type of insect and its caterpillar found in Australia
• Spitfire Wheels, a skateboard-wheel brand by Deluxe Distribution
• Windsor Spitfires, a junior ice hockey team in the Ontario Hockey League
• .22 Spitfire (5.7 x 33 mm) Rifle cartridge
• Fictional future creatures in the TV show The Future Is Wild:
• Spitfire Bird
• Spitfire Beetle
• Spitfire Tree