[spur-mi-deen, -din]
Spermidine is a polyamine involved in cellular metabolism that can be used to stimulate the enzyme, T7 RNA polymerase, a type of RNA polymerase.

Biochemical actions

  • Inhibits neuronal nitric oxide synthase (nNOS).
  • Binds and precipitates DNA.
  • May be used for purification of DNA binding proteins.
  • Stimulates T4 polynucleotide kinase activity.

Some of the uses

  • Can be used in electroporation while transfering the DNA into the cell under the electrical impulse.


Solutions should be sterile-filtered, not autoclaved, if sterile solution is necessary. Spermidine deaminates with time; solutions should be stored frozen. Prepare new solutions frequently.

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