Definitions

Spermidine

Spermidine

[spur-mi-deen, -din]
Spermidine is a polyamine involved in cellular metabolism that can be used to stimulate the enzyme, T7 RNA polymerase, a type of RNA polymerase.

Biochemical actions

  • Inhibits neuronal nitric oxide synthase (nNOS).
  • Binds and precipitates DNA.
  • May be used for purification of DNA binding proteins.
  • Stimulates T4 polynucleotide kinase activity.

Some of the uses

  • Can be used in electroporation while transfering the DNA into the cell under the electrical impulse.

Safety

Solutions should be sterile-filtered, not autoclaved, if sterile solution is necessary. Spermidine deaminates with time; solutions should be stored frozen. Prepare new solutions frequently.

See also

External links

Search another word or see spermidineon Dictionary | Thesaurus |Spanish
Copyright © 2014 Dictionary.com, LLC. All rights reserved.
  • Please Login or Sign Up to use the Recent Searches feature