Spermidine is a polyamine involved in cellular metabolism that can be used to stimulate the enzyme, T7 RNA polymerase, a type of RNA polymerase.

Biochemical actions

• Inhibits neuronal nitric oxide synthase (nNOS).
• Binds and precipitates DNA.
• May be used for purification of DNA binding proteins.
• Stimulates T4 polynucleotide kinase activity.

Some of the uses

• Can be used in electroporation while transfering the DNA into the cell under the electrical impulse.

Safety

Solutions should be sterile-filtered, not autoclaved, if sterile solution is necessary. Spermidine deaminates with time; solutions should be stored frozen. Prepare new solutions frequently.

See also

• Spermine
• Putrescine

External links

• Safety Data Sheet