The melody's first appearance begins with the Munchkins reciting and Judy Garland echoing "Follow the Yellow Brick Road!", which turns into a group vocal by the Munchkins (while Garland skips and dances along the road) and then segues into "You're Off to See the Wizard".
The song occurs as a vocal three more times in the film soundtrack, along with several short instrumental references in the underscore:
Although Jack Haley replaced Ebsen on-screen and in the Tin Man's solo recording of "If I Only Had A Heart", it was deemed unnecessary for the group vocal to be re-recorded, so the voice in the film as released remains Ebsen's. His voice can be detected by listening for the male voice enunciating the "R" in words like "Wizard", as Ebsen's regional accent emphasized the "R" much more strongly than Haley's did.
In The Wizard of Oz and Other Harold Arlen Songs, Shorty Rogers re-worked the song as (in the words of Talkin' Broadway) "a high-energy, wild Latin dance extravaganza".