Prostaglandin-F synthase

(5Z,13E)-(15S)-9alpha,11alpha,15-trihydroxyprosta-5,13-dienoate + NADP⁺ $rightleftharpoons$ (5Z,13E)-(15S)-9alpha,15-dihydroxy-11-oxoprosta-5,13-dienoate + NADPH + H⁺

Thus, the two substrates of this enzyme are (5Z,13E)-(15S)-9alpha,11alpha,15-trihydroxyprosta-5,13-dienoate and NADP⁺, whereas its 3 products are (5Z,13E)-(15S)-9alpha,15-dihydroxy-11-oxoprosta-5,13-dienoate, NADPH, and H⁺.

This enzyme belongs to the family of oxidoreductases, specifically those acting on the CH-OH group of donor with NAD+ or NADP+ as acceptor. The systematic name of this enzyme class is (5Z,13E)-(15S)-9alpha,11alpha,15-trihydroxyprosta-5,13-dienoate:NADP + 11-oxidoreductase. Other names in common use include prostaglandin-D2 11-reductase, reductase, 15-hydroxy-11-oxoprostaglandin, PGD2 11-ketoreductase, PGF2alpha synthetase, prostaglandin 11-ketoreductase, prostaglandin D2-ketoreductase, prostaglandin F synthase, prostaglandin F synthetase, synthetase, prostaglandin F2alpha, prostaglandin-D2 11-reductase, PGF synthetase, NADPH-dependent prostaglandin D2 11-keto reductase, and prostaglandin 11-keto reductase. This enzyme participates in arachidonic acid metabolism.

Structural studies

As of late 2007, 7 structures have been solved for this class of enzymes, with PDB accession codes , , , , , , and .

References

• Reingold DF, Kawasaki A, Needleman P "A novel prostaglandin 11-keto reductase found in rabbit liver". Biochim. Biophys. Acta. 659 179–88.
• Watanabe K, Shimizu T and Hayaishi O "Enzymatic conversion of prostaglandin-D2 to prostaglandin-F2alpha in the rat lung". Biochem. Int. 2 603–610.
• Watanabe K, Yoshida R, Shimizu T, Hayaishi O "Enzymatic formation of prostaglandin F2 alpha from prostaglandin H2 and D2. Purification and properties of prostaglandin F synthetase from bovine lung". J. Biol. Chem. 260 7035–41.
• Wong PY "Purification and partial characterization of prostaglandin D2 11-keto reductase in rabbit liver". Biochim. Biophys. Acta. 659 169–78.
• Wong PY "Purification of PGD2 11-ketoreductase from rabbit liver". Methods. Enzymol. 86 117–25.

External links

The CAS registry number for this enzyme class is .

Gene Ontology (GO) codes