These birds have yellow underparts with dark streaks on the flanks, and olive upperparts with rusty streaks on the back; they have a yellow line above the eye, a dark line through it, and a yellow spot below it. These birds have black legs, long tails, pale wing bars, and thin pointed bills. Coloring is duller in female and immatures.
Their breeding habitats are brushy areas and forest edges in eastern North America. The Prairie Warbler's nests are open cups, which are usually placed in a low area of a tree or shrub.
Prairie Warblers forage actively on tree branches, and sometimes fly around with the purpose of catching insects, which are the main food source of these birds.
Prairie Warblers have two categories of songs, referred to as Type A and Type B. Type A songs are typically a series of ascending buzzy notes. The B songs are an ascending series of whistled notes that often contain some buzzy notes. Compared to A songs, the B songs are lower in pitch, have fewer, longer notes. The total song length is longer as well in Type B songs. The use of these two song categories is associated with certain contexts. A songs are sung throughout the day when males first arrive on their breeding grounds. Once males are paired they begin to sing B songs during the dawn chorus and then will intersperse A songs in their singing during the rest of the day. During this later period of singing A songs are typically used near females, near the nest, and in the center of their territories. In contrast B songs are used when interacting or fighting with other males and near the borders of their territories.
Part of their call note repertoire is a tsip call. During dawn, chorus B songs are interspersed with rapid loud "check" calls.