Any of a group of the smallest known animal viruses. (Pico refers to their small size, rna to their core of RNA.) This group of spheroidal viruses includes viruses that attack the vertebrate intestinal tract and often invade the central nervous system as well (such as polioviruses), viruses that infect tissues in the vertebrate nose (rhinoviruses), and the virus agent of foot-and-mouth disease.

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A Picornavirus is a virus belonging to the family Picornaviridae. Picornaviruses are non-enveloped, positive-stranded RNA viruses with an icosahedral capsid. The genome RNA is unusual because it has a protein on the 5' end that is used as a primer for transcription by RNA polymerase. The name is derived from pico meaning small, and RNA referring to the ribonucleic acid genome, so "picornavirus" literally means small RNA virus.

Picornaviruses are separated into nine distinct genera and include many important pathogens of humans and animals. The diseases they cause are varied, ranging from acute "common-cold"-like illnesses, to poliomyelitis, to chronic infections in livestock. Two main categories are enteroviruses and rhinoviruses.

Classification

Picornaviruses are classed under Baltimore's viral classification system as group IV viruses as they contain a single stranded, positive sense RNA genome of between 7.2 and 9.0 kb in length. Like most positive sense RNA genomes, the genetic material alone is infectious; although substantially less virulent than if contained within the viral particle, the RNA can have increased infectivity when transfected into cells. The genome itself is the same sense as mammalian mRNA, being read 5’ to 3’. Unlike mammalian mRNA Picornaviruses do not have a 5’ CAP but a virally encoded protein known as VPg, however like mammalian mRNA the genome does have a poly A tail at the 3’ end. There is an un-translated region (UTR) at both ends of the Picornavirus genome. The 5’ UTR is longer, being around 600-1200 BP in length, compared to that of the 3’ UTR, which is around 50-100bp. It is thought that the 5’ UTR is important in translation and the 3’ in negative strand synthesis; however the 5’ end may also have a role to play in virulence of the virus. The rest of the genome encodes structural proteins at the 5’ end and non-structural proteins at the 3’ end in a single polyprotein. Experimental data from single step growth-curve-like experiments have allowed scientists to look at the replication of the picornaviruses in great detail. The whole of replication occurs within the host cell cytoplasm and infection can even happen in cells that do not contain a nucleus (known as enucleated cells) and those treated with actinomycin D (this antibiotic would inhibit viral replication if this occurred in the nucleus.)

Structure of the Picornaviruses

The capsid is an arrangement of 60 protomers in a tightly packed Icosahedral structure. Each protometer consists of 4 polypeptides known as VP (viral protein)1, 2, 3 and 4. All of these VP polypeptides originate from one protomer known as VP0 that is cleaved to give the different capsid components. The Icosahedral is said to have a triangulation number of 3, this means that in the icosahedral structure each of the 60 triangles that make up the capsid are split into 3 little triangles with a subunit on the corner. Depending on the type and degree of dehydration the viral particle is around 27-30nm in diameter. The viral genome is around 2500nm in length so we can therefore conclude that it must be tightly packaged within the capsid along with substances such as sodium ions in order to cancel out the negative charges on the RNA caused by the phosphate groups.

Picornavirus replication

The viral particle binds to cell surface receptors. This causes a conformational change in the viral capsid proteins, and myristic acids are released. These acids form a pore in the cell membrane through which RNA is injected Once inside the cell, the RNA un-coats and the (+) strand RNA genome is replicated through a double-stranded RNA intermediate that is formed using viral RDRP (RNA-Dependent RNA polymerase). Translation by host cell ribosomes is not initiated by a 5' G cap as usual, but rather is initiated by an IRES (Internal Ribosome Entry Site). The viral lifecycle is very rapid with the whole process of replication being completed on average within 8 hours. However as little as 30 minutes after initial infection, cell protein synthesis declines to almost zero output – essentially the macromolecular synthesis of cell proteins is “shut off”. Over the next 1-2 hours there is a loss of margination of chromatin and Homogeneity in the nucleus, before the viral proteins start to be synthesized and a vacuole appears in the cytoplasm close to the nucleus that gradually starts to spread as the time after infection reaches around 3 hours. After this time the cell plasma membrane becomes permeable, at 4-6 hours the virus particles assemble, and can sometimes be seen in the cytoplasm. At around 8 hours the cell is effectively dead and lyses to release the viral particles.

History

In 1897, foot-and-mouth disease virus (FMDV), the first animal virus, was discovered. FMDV is the prototypic member of the Aphthovirus genus in the Picornaviridae family. The plaque assay was developed using poliovirus. Both RNA dependent RNA polymerase and polyprotein synthesis were discovered by studying poliovirus infected cells.

Types of Picornavirus

Picornaviruses are separated into nine distinct genera. Contained within the picornovirus family are many organisms of importance as vertebrate and human pathogens, shown in the table below.

Picornavirus Genera, Species and Serotypes

Genus	Species (* signifies type species)	Serotypes
Enterovirus	Bovine enterovirus	two types: bovine enterovirus (BEV) 1 and BEV-2
	Human enterovirus A	21 types including some coxsackie A viruses and enteroviruses
	Human enterovirus B	57 types including enteroviruses, coxsackie B viruses, echoviruses, and swine vesicular disease virus
	Human enterovirus C	14 types including some coxsackie A viruses and enteroviruses
	Human enterovirus D	three types: EV-68, EV-70, EV-94
	Poliovirus *	three types: poliovirus (PV) 1, PV-2 and PV-3
	Porcine enterovirus A	one type: porcine enterovirus (PEV) 8
	Porcine enterovirus B	two types: PEV-9 and PEV-10
	Simian enterovirus A	one type: simian enterovirus (SEV) A1
Rhinovirus	Human rhinovirus A *	74 serotypes
	Human rhinovirus B	25 serotypes
Hepatovirus (also classed as Heparnavirus	Hepatitis A virus *	one serotype: Hepatitis A virus (HAV)
	Avian encephalomyelitis-like viruses	one type: avian encephalomyelitis virus (AEV)
Cardiovirus	Encephalomyocarditis virus *	one serotype: encephalomyocarditis virus (EMCV). Note: Columbia SK virus, Maus Elberfeld virus and Mengovirus are strains of EMCV.
	Theilovirus	five types: Theiler's murine encephalomyellitis virus (TMEV), Vilyuisk human encephalomyelitis virus (VHEV), Theiler-like virus (TLV) of rats, Saffold virus (SAFV) 1 and SAFV-2
Aphthovirus	Foot-and-mouth disease virus *	seven serotypes: O, A, C, Southern African Territories (SAT) 1, SAT 2, SAT 3 and Asia 1
	Equine rhinitis A virus	single serotype: equine rhinitis A virus (ERAV)
Parechovirus	Human parechovirus *	six types: Human parechovirus (HPeV) 1, HPeV-2, HPeV-3, HPeV-4, HPeV-5, HPeV-6
	Ljungan virus	Possibly three types have been described
Erbovirus	Equine rhinitis B virus *	Three types: equine rhinitis B virus (ERBV) 1, ERBV-2, ERBV-3
Kobuvirus	Aichi virus *	single serotype: Aichi virus (AiV)
	Bovine kobuvirus	Single serotype: bovine kobuvirus (BKV)
Teschovirus	Porcine teschovirus *	11 serotypes: porcine teschovirus (PTV) 1 to PTV-11
Sources Picornaviridae. Medical Subject Headings (MeSH). National Library of Medicine. Retrieved on 2007-09-03.. Picornavirus. Institute for Animal Health. Retrieved on 2007-09-03.. ICTVdB - The Universal Virus Database, version 4. Columbia University, New York, USA. (2006). .

Enteroviruses infect the enteric tract as it is visible from its name. On the other hand, rhinoviruses infect primarily the nose and the throat. Enteroviruses replicate at 37°C, whereas Rhinoviruses grow better at 33°C, as this is the lower temperature of the nose. Enteroviruses are stable under acid conditions and thus they are able to survive exposure to gastric acid. In contrast, Rhinoviruses are acid-labile and that is the reason why Rhinoviruses are restricted to the nose and throat.

See also

• Dicistroviridae
• VPg
• Animal viruses

References

External links

• Picornaviruses - description, replication, disease
• Picornaviruses in NCBI Taxonomy browser
• Picornaviridae classification by the International Committee on Taxonomy of Viruses
• Animal viruses