Recognizing his father's failure to capitalize on his proficiency with mechanics and electronics, and not wanting to follow in those footsteps, Nog resolves to go into a career where he can make something of himself. After going through the Ferengi coming of age rite, he requests an apprenticeship under Captain (Commander at the time) Benjamin Sisko in order to attend Starfleet Academy ("Heart of Stone"). Nog is admitted and becomes the first Ferengi in Starfleet ("Facets").
Starfleet assigns Nog to Deep Space Nine as part of his cadet field training ("The Ascent"). Upon returning to the station, he becomes roommates with Jake although his newfound Academy discipline is initially at odds with Jake's slovenly habits. As a cadet, Nog works mainly under chief of operations Miles O'Brien.
In 2374, Nog is sent on a diplomatic mission to Ferenginar with Jake, who is along as a civilian press correspondent. They are rescued by the USS Valiant after their runabout is destroyed by the Dominion. Onboard the Valiant, Jake and Nog discover that the vessel, including all senior officers, is composed entirely of an elite group of Starfleet cadets, called Red Squad. Nog becomes enamored at the thought of working with such a group, and the young Captain soon gives Nog a temporary field promotion and officer position on the ship. An arrogant, overly zealous crew, they embark on a poorly-planned mission to seek out and destroy a very substantial Dominion supply ship... based solely on an untested scientific theory which indicates a specific particle emission could be used to cripple the Dominion ship's hull structure. Jake objects strongly to the recklessness of the endeavor, but is blatantly ignored and ridiculed by everyone, including Nog. Eventually, the Valiant encounters the Dominion ship, and the young crew become visibly shaken when they see how huge this particular enemy ship is, from the viewscreen. They make the first offensive strike on the enemy anyway, but quickly discover to their horror that the theory is flawed. The particle beam has no effect, and the Dominion ship retaliates, resulting in the total destruction of the USS Valiant. Finding an escape-pod, Nog, Jake, and a female cadet whom Jake befriended earlier are the only survivors ("Valiant").
On AR-558, during a battle in the height of the Dominion war, a Jem'Hadar soldier shoots Nog's left leg, necessitating its amputation ("The Siege of AR-558"). The leg is replaced with a bionic one by Dr. Bashir, and Nog chooses Vic Fontaine's holosuite club simulation as the place to recuperate from the trauma of war, and to adjust to using an artificial limb ("It's Only a Paper Moon").
In the Mirror Universe, Nog owns the bar on Terok Nor, having inherited it after the deaths of his father and uncle. The mirror Nog helps Intendant Kira Nerys escape from the Terran Rebellion; however, the Intendant subsequently executes Nog, fearing that he might tell the rebels where she is ("Shattered Mirror").
Nog is known to store his money in a locked box under his bed.