Alkanal monooxygenase (FMN-linked)

RCHO + reduced FMN + O₂ $rightleftharpoons$ RCOOH + FMN + H₂O + hnu

The 3 substrates of this enzyme are RCHO, reduced FMN, and O₂, whereas its 4 products are RCOOH, FMN, H₂O, and hn.

This enzyme belongs to the family of oxidoreductases, specifically those acting on paired donors, with O2 as oxidant and incorporation or reduction of oxygen. The oxygen incorporated need not be derived from O2 with reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor. The systematic name of this enzyme class is alkanal,reduced-FMN:oxygen oxidoreductase (1-hydroxylating, luminescing). Other names in common use include bacterial luciferase, aldehyde monooxygenase, luciferase, and Vibrio fischeri luciferase.

Structural studies

As of late 2007, 4 structures have been solved for this class of enzymes, with PDB accession codes , , , and .

References

• Hastings JW "Bacterial bioluminescence light emission in the mixed function oxidation of reduced flavin and fatty aldehyde". CRC. Crit. Rev. Biochem. 5 163–84.
• Hastings JW, Nealson KH "Bacterial bioluminescence". Annu. Rev. Microbiol. 31 549–95.
• Hastings JW, Presswood RP "Bacterial luciferase: FMNH2-aldehyde oxidase". Methods. Enzymol. 53 558–70.
• Nealson KH, Hastings JW "Bacterial bioluminescence: its control and ecological significance". Microbiol. Rev. 43 496–518.
• Suzuki K, Kaidoh T, Katagiri M and Tsuchiya T "O2 incorporation into a long-chain fatty-acid during bacterial luminescence". Biochim. Biophys. Acta 722 297–301.

External links

The CAS registry number for this enzyme class is .

Gene Ontology (GO) codes