• Hotspot (geology), a location above a hot upwelling plume of mantle.
• Hot Spot, a keno game offered by the California Lottery
• Hot spot (veterinary medicine)

Computer science

• Software framework hot spots describe the variation points in software frameworks.
• Hotspot (Wi-Fi), a Wi-Fi access point or area, in particular for connecting to the Internet
• HotSpot, a Java Virtual Machine from Sun Microsystems
• Screen hotspots, especially in GUI applications, provide areas on which computer-users click or hover for extra information-processing

Biology

• Biodiversity hotspot, a region of significant biodiversity
• Mutation hotspot, DNA positions where mutations occur with above-average frequency
• Recombination hotspot, DNA positions where Genetic recombination occurs with above-average frequency

Arts and entertainment

• Hot Spot (cricket), an infra-red tracking system used in cricket
• Hot Spot (musical), a 1963 musical with lyrics by Martin Charnin and music by Mary Rodgers
• Hot Spot (song), a song by hip-hop rap artist Foxy Brown
• The Hot Spot, a 1990 film with Don Johnson and Jennifer Connelly
• The Hotspot, a weekly podcast by the editors of GameSpot discussing various gaming-related news and events
• Hot Spot (comics), is a fictional character in publications from DC Comics
• Hot Spot (Transformers) is the name of several Transformers characters