Hemolysis (or haemolysis)—from the Greek Hemo-, Greek Αἷμα meaning blood, -lysis, meaning to break open—is the breaking open of red blood cells and the release of hemoglobin into the surrounding fluid (plasma, in vivo).
Most causes of in vitro hemolysis are related to specimen collection. Difficult collections, unsecure line connections, contamination, and incorrect needle size, as well as improper tube mixing and incorrectly filled tubes are all frequent causes of hemolysis. Excessive suction can cause the red blood cells to be literally smashed on their way through the hypodermic needle owing to turbulence and physical forces. Such hemolysis is more likely to occur when a patient's veins are difficult to find or when they collapse when blood is removed by a syringe or a modern vacuum tube. Experience and proper technique are key for any phlebotomist or nurse to prevent hemolysis. In vitro hemolysis can also occur in a blood sample owing prolonged storage or storage in incorrect conditions (ie too hot, too cold).
Hemolytic patterns of the various Gram positive cocci; Streptococci are differentiated by hemolysis of red blood cells on blood agar (BA) plates.
Correction factors for estimating potassium concentrations in samples with in vitro hemolysis: a detriment to patient safety.(Report)
Jun 01, 2009; The preanalytic phase of laboratory medicine is the phase that can most adversely affect the accuracy of test results. (1) Poor...
EDTNA-ERCA Journal Club discussion.(Journal Club Discussion: Guarding Against Hidden Hemolysis During Dialysis: An Overview)(European Dialysis and Transplant Nurses Association/ European Renal Care Association)(Clinical report)
Jan 01, 2008; The paper selected was a case-study paper entitled "hemolysis: A Hidden Danger" by Elisabeth Harman and Paula Dutka ....