• Cardiac cycle of the heart
• Heart sounds, in medicine
• Pulse
• Heartbeat (TV series), a British police drama
• Heartbeat (novel), by Sharon Creech
• Heartbeat (program), clustering software from Linux-HA project
• Heartbeat (company), a video game developer that contributed to the Dragon Quest games

In music:

• Heartbeat Records, a record label
• Heartbeat: The Abbreviated King Crimson, a 1991 compilation album
• Heartbeat (album), an album and an eponymous single by Don Johnson
• The Heartbeats, a 50s doo wop group
• "Heartbeats", song by The Knife
• "Heartbeat" (Paris Hilton song)
• "Heartbeat" (King Crimson song)
• "Heartbeat" (Buddy Holly song)
• "Heartbeat" (Big Black song)
• "Heartbeat" (Steps song)
• "Heartbeat" (Scouting for Girls song)
• "Heartbeat" (Jimmy Somerville song)
• A song by Red 7
• A song by Annie
• A song by Stacey Q