Adenosylcobyric acid synthase (glutamine-hydrolysing)

4 ATP + adenosylcobyrinic acid a,c-diamide + 4 L-glutamine + 4 H₂O $rightleftharpoons$ 4 ADP + 4 phosphate + adenosylcobyric acid + 4 L-glutamate

The 4 substrates of this enzyme are ATP, adenosylcobyrinic acid a,c-diamide, L-glutamine, and H₂O, whereas its 4 products are ADP, phosphate, adenosylcobyric acid, and L-glutamate.

This enzyme belongs to the family of ligases, specifically those forming carbon-nitrogen bonds carbon-nitrogen ligases with glutamine as amido-N-donor. The systematic name of this enzyme class is adenosylcobyrinic-acid-a,c-diamide:L-glutamine amido-ligase (ADP-forming). Other names in common use include CobQ, cobyric acid synthase, 5'-deoxy-5'-adenosylcobyrinic-acid-a,c-diamide:L-glutamine, amido-ligase, and Ado-cobyric acid synthase [glutamine hydrolyzing]. This enzyme participates in porphyrin and chlorophyll metabolism.

References

• Blanche F, Couder M, Debussche L, Thibaut D, Cameron B, Crouzet J "Biosynthesis of vitamin B12: stepwise amidation of carboxyl groups b, d, e, and g of cobyrinic acid a,c-diamide is catalyzed by one enzyme in Pseudomonas denitrificans". J. Bacteriol. 173 6046–51.
• Warren MJ, Raux E, Schubert HL, Escalante-Semerena JC "The biosynthesis of adenosylcobalamin (vitamin B12)". Nat. Prod. Rep. 19 390–412.

External links

Gene Ontology (GO) codes