In upstate New York and southern Ontario the sedimentary rocks tend to slope slightly to the southward, and the Onondaga outcrops in a line that usually forms an escarpment (the steep face of a cuesta), because of its resistance to erosion. The outcrop can be traced from the Hudson River valley westward along the southern rim of the Mohawk River valley, passing just south of Syracuse, and along the northern heads of the major Finger Lakes to Buffalo, New York. From Fort Erie, Ontario it runs to Windsor just north of the Lake Erie shoreline, becoming less prominent as one travels westward. It is not distinct west of Windsor, but begins to become noticeable as a steep hill just northwest of Leamington, as it forms a low ridge/escarpment along much of the Lake Erie shoreline.
In several spots it is breached by geologically young streams and spectacular waterfalls are formed, such as at Chittenango Falls just east of Syracuse, Buttermilk Falls at Le Roy, New York and Indian Falls west of Batavia.
A few other breaches occur in older valleys, which likely once had waterfalls, but erosion eventually obliterated them. Such breaches occur at the Tully valley, the Genesee River valley near Avon, New York, and at Port Colborne, Ontario, where the old valley forms a harbor on Lake Erie.
The formation is broken by the only major fault line in western New York, the Linden Fault just east of Batavia, where the eastern side of the fault has dropped down and the ledge moved southward relative to the western side. On the western side of the fault in Genesee County the escarpment achieves its greatest prominence. The New York State Thruway has a rock cut at Batavia which clearly shows the fault and is a popular point for geology class field trips. The fault, which runs from Attica, New York northward to Lake Ontario, is still active and periodically causes minor earthquakes in the area.
A similar and more prominent outcrop known as the Niagara Escarpment runs parallel and about 25 miles (40 kilometers) to the north through upstate New York, but curves northwestward in southern Ontario toward Lake Huron and eventually into Michigan's Upper Peninsula and Wisconsin's Door Peninsula.
Another smaller outcrop known as the Portage Escarpment lies about 35 miles to the south, running along the southern ends of the Finger Lakes and forming Cascadilla, Ithaca and Buttermilk Falls in Ithaca.
The Onondaga Escarpment contains significant outcrops of a type of chert (flint) which bears the escarpment's name. This variety of chert was of great importance to First Nations peoples throughout Southern Ontario, who used it to make stone tools (lithics) such as projectile points and hide scrapers. This variety of chert, which is of reasonably high-quality and which was highly-valued by First Nations peoples, is often a common variety of chert recovered archaeologically from sites relatively adjacent to outcrops; for example, Onondaga-variety chert comprises 95% of all of the flint material from some sites in Milton, Ontario. The material has also been found as well at some distance from its original source; Onondaga chert has been recovered at the late archaic Duck Lake archaeological site in northern Michigan, circa 400 kilometers from the nearest outcropping of the material. This wide distribution implies either a very large seasonal migration of ancient peoples or long-distance trade routes, with both likely being the case at different times throughout the prehistory of the Great Lakes region.