In an experimental setting, Fc and Fab fragments can be generated in the laboratory. The enzyme papain can be used to cleave an immunoglobulin monomer into two Fab fragments and an Fc fragment. The enzyme pepsin cleaves below hinge region, so a F(ab')2 fragment and a Fc fragment is formed. The variable regions of the heavy and light chains can be fused together to form a single chain variable fragment (scFv), which is only half the size of the Fab fragment yet retains the original specificity of the parent immunoglobulin.
WIPO ASSIGNS PATENT TO INSTITUTE OF MEDICLNAL BIOTECHNOLOGY, CHLNESE ACADEMY OF MEDICAL SCIENCES, LNSTITUTE OF HEMATOLOGY, CHINESE ACADEMY OF MEDICAL SCIENCES & PEKING UNLON MEDICAL COLLEGE FOR "FUSION PROTEIN OF ANTI-CD20 ANTIBODY FAB FRAGMENT AND LIDAMYCIN, PREPARATION METHOD AND USE THEREOF" (CHINESE INVENTORS)
Feb 08, 2011; GENEVA, Feb. 8 -- Publication No. WO/2011/011973 was published on Feb. 03. Title of the invention: "FUSION PROTEIN OF ANTI-CD20...