• East Brunswick Township, New Jersey, a small city in central New Jersey
• EarthBound, a role-playing game for the Super Nintendo
• EB (beer), Polish beer
• EB (train), the cabless second unit of an EMD EA/EB diesel locomotive
• Eiðis Bóltfelag, a Faroese football club from the village of Eiði
• Ekstra Bladet, a Danish tabloid newspaper
• Electric Boat, a major builder of submarines for the United States Navy
• Electron beam, a stream of electrons observed in vacuum tubes
• Electronic Business
• Electronics Boutique, a computer and video games retailer
• Elektrobit, a Finnish company in the wireless and automotive business.
• Elijah Burke, an American professional wrestler
• Encyclopædia Britannica
• Enterprise Bargaining, a workplace process often called collective bargaining
• Epidermolysis bullosa, a rare genetic disease
• Epstein-Barr virus
• Ethidium bromide, an agent commonly used in molecular biology
• Europa Barbarorum, a modification of the computer game Rome: Total War
• European Baccalaureate, a degree given on completion of the last year of attendance at the European School
• Evans Blue, a Canadian alternative rock band
• Exabit (Eb), a unit of information used, for example, to quantify computer memory or storage capacity
• Exabyte (EB), a unit of information used, for example, to quantify computer memory or storage capacity
• Exclusive Brethren, a Christian Evangelical religious movement

Eb could also refer to:

• the note, and subsequent keys of and relating to E-flat