The original members of the Disco Biscuits met as undergraduate students at the University of Pennsylvania. Gutwillig and Altman became friends and began to play music together. Once Brownstein joined the group, they began to play at college parties and bars throughout Pennsylvania. They called themselves different names at every show until they settled on the Disco Biscuits. Disco Biscuit set-lists consist of mostly original songs which can be played inverted, unfinished, or "dyslexic".
In 1999, the first Camp Bisco was held in Pennsylvania, which would grow to be their flagship festival, hosting such artists as Snoop Dogg, The Roots, STS9, Thievery Corporation and Umphrey's McGee. In early 2000, Brownstein left the band. On July 12,2000 at the Crowbar in State College, PA the band announced that Brownstein was back in the band saying simply that "There is no Disco Biscuits without Marc Brownstein".
In 2002 lighting designer Johnny R Goode III (R Goode) joined the organization. A long time fan and friend to the band, R Goode was previously Lighting Designer for the Skydog Gypsy (Aucoin's former band).
After another year on the East Coast, the band then relocated to Santa Cruz, CA. Their 2002 album Señor Boombox charted on two Billboard Magazine entries: Top Electronic Albums at #13 and Top Independent Albums at #46.
Marc helped create the HeadCount organization in 2004 when the Biscuits were not touring regularly, and was involved in every facet of the organization. Marc continues to be involved in all key decisions related to HeadCount and serves as a liaison with other HeadCount-affiliated artists.
In 2005 at Camp Bisco IV in Van Etten, NY, at the Skyetop Festival Ground, Altman left the band to attend medical school and in the following fall, Allen Aucoin was announced as Altman's replacement at drums.
The band has toured nationally as well as played at major music festivals such as Jam on the River in Philadelphia, Pennsylvania, Bonnaroo in Manchester, Tennessee, Gathering of the Vibes in Bridgeport, Connecticut, All Good Music Festival in Masontown, West Virginia, Lollapalooza 2006, Jam Cruise, Rothbury in Rothbury, Michigan, and a New Years Eve show with support by Keller Williams, ?uestlove and Shpongle at the Tweeter Center in Camden, New Jersey, and the 10,000 Lakes Festival in Detroit Lakes, Minnesota.
In 2007, the band co-headlined the D.U.M.B. (Disco-Umphrey's McGee-Biscuits) tour with Umphrey's McGee.
Drummer Allen Aucoin has also played a few shows in a project with Brock Butler from Perpetual Groove throughout 2008.
The band has been in the studio for the past year changing gears a little. They have been drawing on influences from high profile hip hop Philly producers like Don Cheegro and Dirty Harry, who recently wrote beats for Ludacris, Fantasia, Chris Brown and Beanie Sigel. “We’ve been sitting in the throng of the hip hop community,” said Brownstein.