• Ballet tutu, a type of costume for ballet performances
• Tutu (plant), poisonous New Zealand plants of the genus Coriaria
• Tutu (album), a 1986 album by Miles Davis
• Tūtū, a composition by Liliuokalani of Hawaii
• Tutu de Feijão, a paste made of beans and manioc flour in Southeastern Brazilian cuisine
• Tutu, a mod for the computer game Baldur's Gate which allows playing the first game of the series within the more advanced engine of the second one

Figures in mythology or popular culture:

• Tutu (god), in Mesopotamian mythology, one of the Anunnaku
• Princess Tutu, an anime series or the character in that series
• The wife of Tottles the Bear

Places:

• Tutu, U.S. Virgin Islands, a subdivision of the island of Saint Thomas.

People:

• Osei Kofi Tutu I (died 1717), Ashantehene, ruler of Kumasi, co-founder of the Empire of Ashanti
• Desmond Tutu (born 1931), South African Archbishop, activist against apartheid, Nobel Peace Prize winner
• Leah Nomalizo Tutu, wife of Desmond Tutu