Born 3 April 1778 at Saint-Georges-sur-Cher, in the Loir-et-Cher département. His father was a surgeon. He studied with his uncle, the vicar at Chenonceaux (Indre-et-Loire) department along with the children of the Chenonceau château. Madame Dupin the grandmother of George Sand, financed his medical studies in Paris.
He married Madame Dupin's lecturer and settled in Renaudière in Chenonceaux (the Renaudière is currently a restaurant and hotel). Very curious and clever, he had a laboratory at his disposal and occupied himself with gardening and other manual labours in his spare time.
He was the mayor of Chenonceaux from 1803 to 1807. He spent 15 years at Chenonceaux gaining experience, wrote his thesis in medicine in 1815 and then became medical director at the hospital in Tours; which currently bears his name. He continued his study of disease and founded the medical school at Tours.
Dr. Bretonneau died in 1862. He is buried in Saint-Cyr-sur-Loire, near Tours.
He performed the first successful tracheotomy in 1825, distinguished between scarlet fever and diphtheria in 1826. He studied disease in detail and was the first to think that disease was caused by bacteria in 1855, however, a microscope was not available to him and he was unable to confirm his hypothesis. He also discovered that the same illness could manifest itself differently in different patients. It was the beginning of scientific medicine: where careful observation is used to find cures for sickness and solutions to problems.