is an image on an x-ray
film or nuclear emulsion produced by the pattern of decay emissions (e.g., beta particles
or gamma rays
) from a distribution of a radioactive
substance. Alternatively, the autoradiograph can also be available as a digital image (digital autoradiography), thanks to the recent development of scintillation gas detectors or rare earth phosphorimaging systems. In biology, this technique may be used to determine the tissue localization of a radioactive substance, either introduced into a metabolic pathway, bound to a receptor or enzyme, or hybridized to a nucleic acid. The film or emulsion is apposed to the labeled tissue section to obtain the autoradiograph (also called an autoradiogram). The auto-
prefix indicates that the radioactive substance is within the sample, as distinguished from the case of historadiography
or microradiography, in which the sample is X-rayed using an external source.
The use of radiolabeled ligands to determine the tissue distributions of receptors is termed either in vivo or in vitro receptor autoradiography if the ligand is administered into the circulation (with subsequent tissue removal and sectioning) or applied to the tissue sections, respectively. The ligands are generally labeled with 3H (tritium) or 125I.
The distribution of RNA transcripts in tissue sections by the use of radiolabeled, complementary oligonucleotides or ribonucleic acids ("riboprobes") is called in situ hybridization histochemistry. RNA or DNA viral sequences can also be located in this fashion. These probes are usually labeled with 32P, 33P, or 35S.
This autoradiographic approach contrasts to techniques such as PET and SPECT where the exact 3-dimensional localization of the radiation source is provided by careful use of coincidence counting, gamma counters and other devices.
- Andrew W. Rogers. Techniques of Autoradiography, Elsevier North Holland, 3rd edition, 1979.