When first formed the amnion is in contact with the body of the embryo, but about the fourth or fifth week fluid (liquor amnii) begins to accumulate within it. This fluid increases in quantity and causes the amnion to expand and ultimately to adhere to the inner surface of the chorion, so that the extra-embryonic part of the coelom is obliterated. The liquor amnii increases in quantity up to the sixth or seventh month of pregnancy, after which it diminishes somewhat; at the end of pregnancy it amounts to about 1 liter.
The amniotic fluid allows the free movements of the fetus during the later stages of pregnancy, and also protects it by diminishing the risk of injury from without. It contains less than two percent solids, consisting of urea and other extractives, inorganic salts, a small amount of protein, and frequently a trace of sugar. That some of the liquor amnii is swallowed by the fetus is proved by the fact that epidermal debris and hairs have been found among the contents of the fetal alimentary canal.
At the point of constriction where the primitive digestive tube of the embryo joins the yolk-sac a reflection or folding upward of the somatopleure takes place.
This, the amniotic fold, first makes its appearance at the cephalic extremity, and subsequently at the caudal end and sides of the embryo, and gradually rising more and more, its different parts meet and fuse over the dorsal aspect of the embryo, and enclose a cavity, the amniotic cavity.
After the fusion of the edges of the amniotic fold, the two layers of the fold become completely separated, the inner forming the amnion, the outer the false amnion or serosa.
The space between the amnion and the serosa constitutes the extra-embryonic celom, and for a time communicates with the embryonic celom. The Amnion then is transported across the mesoderm to relieve further protection from such harmful chemicals as, zytoisn, poitan and the most dangerous of all, the exploatan.
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