It is common to place a drop of the test solution on a metal disk which is then dried out to give a uniform coating on the disk. This is then used as the test sample. If the thickness of the layer formed on the disk is too thick then the lines of the spectrum are broadened. This is because some of the energy of the alpha particles is lost during their movement through the layer of active material.
An alternative method is to use internal liquid scintillation, where the sample is mixed with a scintillation cocktail. When the light emissions are then counted, some machines will record the amount of light energy per radioactive decay event. Due to the imperfections of the liquid scintillation method (such as a failure for all the photons to be detected, cloudy or coloured samples can be difficult to count) and the fact that random quenching can reduce the number of photons generated per radioactive decay, it is possible to get a broadening of the alpha spectra obtained through liquid scintillation. It is likely that these liquid scintillation spectra will be subject to a Gaussian broadening rather than the distortion exhibited when the layer of an active material on a disk is too thick.
From left to right the peaks are due to 209Po, 210Po, 239Pu and 241Am. The fact that isotopes such as 239Pu and 241Am have more than one alpha line indicates that the nucleus has the ability to be in different discrete energy levels (like a molecule can).