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AUTORADIOGRAPHY - 2 reference results
MBF Bioscience Stereology
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Autoradiography
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Autoradiograph
An autoradiograph is an image on an x-ray film or nuclear emulsion produced by the pattern of decay emissions (e.g., beta particles or gamma rays) from a distribution of a radioactive substance. Alternatively, the autoradiograph can also be available as a digital image (digital autoradiography), thanks to the recent development of scintillation gas detectors or rare earth phosphorimaging systems. In biology, this technique may be used to determine the tissue localization of a radioactive substance, either introduced into a metabolic pathway, bound to a receptor or enzyme, or hybridized to a nucleic acid. The film or emulsion is apposed to the labeled tissue section to obtain the autoradiograph (also called an autoradiogram). The auto- prefix indicates that the radioactive substance is within the sample, as distinguished from the case of historadiography or microradiography, in which the sample is X-rayed using an external source.

The use of radiolabeled ligands to determine the tissue distributions of receptors is termed either in vivo or in vitro receptor autoradiography if the ligand is administered into the circulation (with subsequent tissue removal and sectioning) or applied to the tissue sections, respectively. The ligands are generally labeled with 3H (tritium) or 125I. The distribution of RNA transcripts in tissue sections by the use of radiolabeled, complementary oligonucleotides or ribonucleic acids ("riboprobes") is called in situ hybridization histochemistry. RNA or DNA viral sequences can also be located in this fashion. These probes are usually labeled with 32P, 33P, or 35S.

This autoradiographic approach contrasts to techniques such as PET and SPECT where the exact 3-dimensional localization of the radiation source is provided by careful use of coincidence counting, gamma counters and other devices.

References

Additional References

  1. Andrew W. Rogers. Techniques of Autoradiography, Elsevier North Holland, 3rd edition, 1979.

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MBF Bioscience Stereology
The most-cited stereology system in the world. Try it now.
www.mbfbioscience.com
Autoradiography
Cassettes and accessories. Browse our inventory and order online.
Sponsored Results www.gelifesciences.com
Intratumoral Spatial Distribution of Hypoxia and Angiogenesis Assessed by ^sup 18^F-FAZA and ^sup 125^I-Gluco-RGD Autoradiography/ REPLY
Oct 01, 2008; TO THE EDITOR: With great interest, we read the paper by Picchio et al. (1). The authors applied a double-tracer autoradiography...
Read more with a free trial on HighBeam.com »
autoradiography
Jan 01, 2004; autoradiography An experimental technique in which a radioactive specimen is placed in contact with (or close to) a photographic...
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STORM(TM) 820 SYSTEM OFFERS FILMLESS AUTORADIOGRAPHY, UPGRADABILITY
Jan 05, 1996; SUNNYVALE, Calif., Jan. 5 /PRNewswire/ -- Molecular Dynamics' (Nasdaq: MDYN) new Storm(TM) 820 system offers quantitative...
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Lognormal Distribution of Cellular Uptake of Radioactivity: Statistical Analysis of [alpha]-Particle Track Autoradiography
Jun 01, 2008; Recently, the distribution of radioactivity among a population of cells labeled with ^sup 210^Po was shown to be well described...
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Imaging of striatal dopamine D(2) receptors with a PET system for small laboratory animals in comparison with storage phosphor autoradiography: A validation study with (18)F-(N-methyl)benperidol
Nov 01, 2001; Several groups have developed high-resolution PET systems and shown the feasibility of in vivo studies on small laboratory...
Read more with a free trial on HighBeam.com »
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