Cyclic adenosine monophosphate
, cyclic AMP
or 3'-5'-cyclic adenosine monophosphate
) is a second messenger
that is important in many biological processes. cAMP is derived from adenosine triphosphate
(ATP) and used for intracellular signal transduction
in many different organisms.
Synthesis and decomposition
cAMP is synthesised from ATP by adenylyl cyclase
which is located at the cell membranes. Adenylyl cyclase is activated by a range of signaling molecules through the activation of adenylyl cyclase stimulatory G (Gs
)-coupled receptors and inhibited by agonists of adenylyl cyclase inhibitory G (Gi
)-protein coupled receptors. Liver adenylyl cyclase responds more strongly to glucagon, and muscle adenylyl cyclase responds more strongly to adrenaline.
cAMP decomposition into AMP is catalyzed by the enzyme phosphodiesterase.
cAMP is a second messenger, used for intracellular signal transduction, such as transferring the effects of hormones
, which cannot get through the cell membrane. Its purposes include the activation of protein kinases
and regulating the effects of adrenaline and glucagon. It is also used to regulate the passage of Ca2+
through ion channels
cAMP and its associated kinases function in several biochemical processes, including the regulation of glycogen, sugar, and lipid metabolism.
In humans, cyclic AMP works by activating protein kinase A (PKA, also known as cAMP-dependent protein kinase). This is normally inactive as a tetrameric holoenzyme, consisting of 2 catalytic and 2 regulatory units (C2R2), with the regulatory units blocking the catalytic centers of the catalytic units. Cyclic AMP binds to specific locations on the regulatory units of the protein kinase, and causes dissociation between the regulatory and catalytic subunits, thus activating the catalytic units and enabling them to phosphorylate substrate proteins.
The active subunits catalyze the transfer of phosphate from ATP to specific serine or threonine residues of protein substrates. The phosphorylated proteins may act directly on the cell's ion channels, or may become activated or inhibited enzymes. Protein kinase A can also phosphorylate specific proteins that bind to promoter regions of DNA, causing increased expression of specific genes. Not all protein kinases respond to cAMP: several types of protein kinases are not cAMP dependent, for example protein kinase C.
Further effects depend on cAMP-dependent protein kinase, which vary based on the type of cell.
, the level of cAMP varies depending on the medium used for growth. In particular, cAMP is low when glucose is the carbon source. This occurs through inhibition of the cAMP-producing enzyme, adenylyl cyclase, as a side effect of glucose transport into the cell. The transcription factor cAMP receptor protein
(CRP) also called: CAP (Catabolite gene Activator Protein) forms a complex with cAMP and thereby is activated to bind to DNA. CRP-cAMP increases expression of a large number of genes, including some encoding enzymes
that can supply energy independent of glucose.
An example of cAMP's function is the positive regulation of the lac operon. In an environment of a low glucose concentration, cAMP accumulates and binds to the allosteric site on CRP (cAMP receptor protein), a transcription activator protein. The protein assumes its active shape and binds to a specific site beside the lac promoter, making it easier for RNA polymerase to bind to the adjacent promoter to start transcription of the lac operon, increasing the rate of lac operon transcription. With a high glucose concentration, the cAMP concentration decreases, and the CRP disengages from the lac operon.
In the species Dictyostelium discoideum
specifically, the chemotactic
movement of cells are organized by periodic waves of cAMP that propagate through the cell. The waves are the result of a regulated production and secretion of extracellular cAMP and a spontaneous biological oscillator that initiates the waves at centers of territories.
Role of cAMP in human carcinoma
Some research has suggested that a deregulation of cAMP pathways and an aberrant activation of cAMP-controlled genes is linked to the growth of some cancers.
Role of cAMP in Prefrontal Cortex Disorders
Recent research may indicate that cAMP affects the function of higher order thinking in the prefrontal cortex
through its regulation of ion channels called hyperpolarization-activated cyclic nucleotide-gated channels
(HCN). When cAMP stimulates the HCN, these gates open, rendering the brain cell closed to communication, thus interfering with prefrontal cortex
function. This research is of interest to scientists studying the brain, especially the degradation of higher cognitive function in ADHD