The arthropod head problem is popularly known as the "endless dispute", the title of a famous paper on the subject by Jacob G. Rempel in 1975, referring to its apparently intractable nature. Although some progress has been made since that time, the precise nature of especially the labrum and the pre-oral region of arthropods remain highly controversial.
A typical insect head possesses a pair of antennae; eyes; mandibles, labrum, maxillae and labium (the latter four forming the cluster of "mouth parts", no. 32. in the diagram). Lying above the oesophagus is the brain or supraesophageal ganglion, divided into three pairs of ganglia: the protocerebrum, deutocerebrum and tritocerebrum from front to back (collectively no. 5 in the diagram). Nerves from the protocerebrum lead to the large compound eyes; from the deuterocerebrum to the antennae; and from the tritocerebrum to the labrum and stomatogastric nervous system. Circum-oesophageal connectives lead from the tritocerebrum around the gut to connect the brain to the ventral ganglionated nerve cord: nerves from the first three pairs of ganglia lead to the mandibles, maxillae and labium, respectively. The position of the mouth and the circum-oesophageal connectives allows a distinction to be made between pre- and post-oral structures; although it should be borne in mind that because structures can move around during development, a pre-oral position of a structure in the adult does not necessarily prove that its developmental origin is from there. The myriapod head is very similar to that of the insects.
The crustacean head is broadly similar to that of the insects, but possesses, in addition, a second pair of antennae that are innervated from the tritocerebrum. In place of the labium, crustaceans possess a second pair of maxillae.
Chelicerate head structures differ considerably from those of mandibulates (i.e. insects, crustaceans and myriapods); they possess eyes and a single pair of grasping appendages innervated from the brain, plus a labrum-like structure. Behind the mouth lies another pair of mouthparts, the pedipalps, and behind them lie the series of walking limbs. In chelicerates, the leg-bearing segments are fused with the anterior segments to form a prosoma, so that in living arthropods a distinct head only exists in mandibulates.
The arthropod head problem has until recently been predicated on the Articulata theory, i.e. that the arthropods and annelids are close relatives. Although arthropods are essentially direct developers that do not possess a trochophore-like larva, the annelids do. During annelid metamorphosis, segments are added close to the posterior of the body, behind the mouth; whereas the brain is derived from the episphere or region in front of the mouth. Recognition of this led to the concept of a primary, non-segmental component of the body in annelids known as the acron being developed, from which the brain is ultimately derived. Because the arthropod and annelid heads, in the light of the Articulata theory, were assumed to be structurally homologous in some way, the arthropod head was also often considered to incorporate a non-segmental acronal component. Taking the homology between annelid and arthropod heads at face value, Swedish workers such as Hanström and Holmgren assumed that a large part of the arthropod head must correspond to the acron, a view followed later by several prominent American insect workers such as Butt and Snodgrass. They proposed that all pre-oral structures in insects were non-segmental, although such a view is at odds with the preoral position of apparently bona fide appendages such as the antennae. A less extreme set of theories propose that only the protocerebrum and associated structures should be considered to be acronal.
The view that the arthropod head must contain an acronal remnant has been shaken by the relatively recent revision of protostome phylogeny, which has dismantled the Articulata and placed the arthropods together with a group of unsegmented worms often referred to as the Cycloneuralia in the so-called Ecdysozoa. All members of the Ecdysozoa are direct developers without a trochophore, and the cycloneuralians have terminal mouths. As a result, the idea of the arthropods having inherited a preoral acron from their ancestors seems less likely.
The study of how developmental genes are expressed during embryogenesis has become an important new tool in the last twenty years for understanding the structure and evolution of morphology. The arthropod head problem has been tackled in three main ways in this regard, first by using genetic segmental markers to probe the obscure region in front of the mouth, especially in insects; second by Hox gene, showing evidence of being evovled in the Paleozoic era, have the ability to express patterns of homology between different arthrods. Just as Hox genes express patterns, Mads-box genes have the potential to initiate an important role in the integration of sexual expression time pathways in plants." Third, by studying gene expression in particular features (especially the labrum) to determine its appendiculate or other status. Because all arthropods have the same complement of nine Hox loci, the morphological diversification observed is caused by these genes being expressed at different areas and times.
It is widely agreed that the insect, myriapod and crustacean heads are very similar. The apparent lack of a second antenna in insects and myriapods is explained by the idea that this appendage has been lost, leaving an appendage-less segment known as the intercalary segment. Modern phylogenies do not in general support an insect-myriapod relationship, suggesting that the second antenna has been lost independently in each group, perhaps as a result of a convergent adaptation to life on land. Furthermore, there is general agreement that the mandibles, first maxillae and labium/second maxillae each represent a post-oral segment; and that the first antenna represents a preoral segment.
Areas of disagreement can be grouped into three categories: the nature of the pre-antennal region in mandibulates; the nature of the labrum; and the relationship between the chelicerate and mandibulate anterior segments.
The degree to which the area in front of the mouth is segmented remains one of the major controversies in the arthropod head problem. As already mentioned, earlier workers often considered the entire pre-oral region to be "acronal" and thus nonsegmental. Modern workers universally accept that at least the deuterocerebrum is segmental. However, the nature of the region in front of this is much less certain. Some molecular development studies have given limited support to the idea of an "ocular" segment corresponding to the protocerebrum; but these data are not unequivocal. The idea of the protocerebrum actually comprising two segments has also received support from both molecular and embryological data.
The labrum is a flap-like structure that lies immediately in front of the mouth in almost all extant euarthropods, the general exception being provided by the probable chelicerate-relatives the pycnogonids. It has proved to be by far the most controversial of all arthropod head structures. It is innervated in crustaceans and insects from the tritocerebrum, i.e. the back of the brain. However, in development it often appears at the anterior of the head, and migrates backwards towards its adult position. Furthermore, it often appears as a bilobed structure, with a set of muscles, nerves and gene expression in many ways similar to that of an appendage. This evidence has been used to suggest that the labrum is in fact a highly reduced appendage. Its innervation from the rear of the brain has suggested to some workers that, if an appendage, it is the appendage of the tritocerebral segment; a point disputed by others who argue that the presence of a well-developed appendage in at least crustaceans in this segment (i.e., the second antenna, corresponding to the intercalary segment of insects) rules this out. If the labrum is an appendage then, it seems possible that its origin is indicated by its developmentally anterior position, i.e., that it is the appendage of a segment anterior to the first antenna. The most obvious choice for this is the segment whose ganglion is the protocerebrum, which in extant euarthropods bears no appendage (apart from the eyes). If the labrum is really an anterior appendage that has migrated to the posterior, then it may be homologous to the "antennae" of onychophorans which, as discussed below, seem to be innervated from a very anterior part of the brain, ie in front of the eyes. It has even been suggested (e.g., by Roonwal) that the labrum belongs to an even more obscure segment that lies in front of the ocular one. Nevertheless, many workers continue to be highly skeptical about the appendiculate nature of the labrum, preferring to see it as it appears, i.e., as an outgrowth of the body wall just in front of the mouth.
The classical view was that the chelicerae were homologous to the second antennae of crustaceans (i.e., they are innervated from the tritocerebrum), a view based partly on the fact that the chelicerae were innervated from the same ganglion that innervates the labrum, which is the tritocerebrum in crustaceans and insects. Given that there are apparently no appendages in front of the chelicerae, the implication was that the deuterocerebrum had been lost in chelicerates (the protocerebrum innervates the eyes in both groups in this view). Whilst this view still has its defenders (notably Collete and Jacques Bitsch), the alternative view that the chelicerae are innervated from the deuterocerebrum has gained ground, based on molecular development in mites and spiders, and neuroanatomy in Limulus. If this is the case, then chelicerates simply have no tritocerebrum, ie there is no third supraoesophageal ganglion of the brain; the segment corresponding to it would be the suboesophageal pedipalp one. Such a theory does not, however, immediately account for the same ganglionic innervation of the chelicerae and labrum, although one solution is simply to claim that the labrum itself is not homologous between mandibulates and chelicerates (the view, for example, of Dieter Waloszek and colleagues).
The brain of onychophorans has been recently re-investigated and shown to possess two unusual features. First, although the mouth is ventral, as is the case in euarthropods, it is innervated from three different places; the sides, the posterior, and by a nerve that originates dorsally, and passes anteriorly down to curve back to the front of the mouth. This set of innervation makes sense if the mouth of onychophorans was originally terminal and has been bent downwards. Second, the antennae of the onychophorans appear to be innervated from in front of the eyes; which in euarthropod terms implies a protocerebral (or potentially even more anterior) innervation. As all euarthropod antennae are deuterocerebral or tritocerebral, this implies that the onychophoran antennae are not homologous to any euarthropod ones.
The Cambrian fossil record, above all the various lagerstätten such as the Burgess Shale, Sirius Passet, Chengjiang and Orsten faunas, has yielded a very rich record of well-preserved arthropods, including the well-known trilobites.
Many Cambrian arthropods, including the trilobites themselves, possess a single pair of slender antennae, which have been equated with either the first or second antennae of the crustaceans; and either the chelicerae of the missing appendages of the supposedly reduced deuterocerebrum in chelicerates. However, another group of arthropods, the so-called "great appendage" arthropods, including Yohoia, Leanchoilia and Alalcomenaeus, do not possess simple antennae, but rather have a robust, branched structure, which was called the "great appendage" by Harry B. Whittington in his restudy of these taxa. Yet another group of arthropods may possess two differentiated head appendages, of which the most important and controversial is the Chengjiang form Fuxianhuia. Fuxianhuia was claimed to possess a pair of short antennae anterior, followed by a robust pair of "sub-chelate" appendages. However, this assessment has been both disputed by Waloszek and colleagues, who consider that the sub-chelate appendages are in fact gut diverticulae; and supported by Graham Budd. Thus, its nature remains controverted at present. Other taxa have also been claimed to have a somewhat similar anterior appendage arrangement (e.g. Fortiforceps) but, with the exception of the well-preserved Branchiocaris from the Burgess Shale, most of them are highly equivocal.
In almost all Cambrian arthropods, the post-oral limbs show very little differentiation compared to the trunk limbs; the heads posterior to the mouth shows a considerable degree of variability, however, in the number of segments incorporated into the head.
Trilobites, in particular, possess a ventral sclerotised plate in the head called the hypostome. Whether this is homologous to the labrum or not is debated; although Waloszek and others have argued that as the phosphatocopines (upper stem-group crustaceans) seem to possess both, it cannot be.
There are at least four main theories to account for anterior head appendages in Cambrian arthropods:
Gerhard Scholtz and Greg Edgecombe accept that the antennae of onychophorans are protocerebral, and call them "primary" antennae to distinguish them from the "secondary" antennae of groups such as the insects and crustaceans. They also accept that taxa such as Fuxianhuia possess both antennae and "great appendages". Because in Fuxianhuia the antennae lie anterior to the great appendages, they suggest that these antennae are the inherited primitive "primary" antennae; and that the great appendages are thus equivalent to the first antennae of crustaceans. Because the secondary antennae are not present in stem group arthropods such as Fuxianhuia, nor in the extant chelicerates, they propose that arthropods, such as the trilobites, that possess secondary antennae, belong in a monophyletic group that also includes the mandibulates, called the Antennata. The trilobites are thus, in their view, not stem-group chelicerates, a commonly-held view, but rather, stem-group mandibulates. The status of the labrum is not resolved by this theory, but they argue that it the evidence for it being appendiculate is not compelling; thus it does not have to correspond to a well-developed appendage of any Cambrian arthropod.
Graham Budd's theory agrees with that of Scholtz and Edgecombe in accepting the protocerebral nature of the onychophoran antennae, and the two preoral appendages of Fuxianhuia. However, he traces the origin of the "great appendages" in the differentiated frontal appendages of Cambrian lobopods such as Aysheaia and Kerygmachela, neither of which possess convincing antennae. Thus, in Budd's view, the order of the two anterior appendages of taxa such as Fuxianhuia are reversed: the antennae are the first antennae (deutocerebral) of the mandibulates; and the great appendages correspond to the primary antennae of the onychophorans and Cambrian lobopods. Following previous work by Dewel and colleagues, Budd accounts for their reversal by arguing that the mouth in basal lobopods was terminal, and that as it rotated backwards and downwards, it brought the anterior appendage backwards with it. Given this transformation, it is likely, under this theory, that the remnant of the great appendage/primary antenna is the labrum of extant arthropods. Because in this view Fuxianhuia possesses both a hypostome and a great appendage, the hypostome cannot be straightforwardly homologous with the labrum.
Maxmen and others recently published a morphologically-based paper that claimed the enigmatic chelifores of extant pycnogonids are innervated from the protocerebrum, and not from the trito- or deutocerebrum as previously claimed. This would suggest that pycnogonids had uniquely retained a "great appendage" homologue as an appendage, unlike all other euarthropods in which it had been transformed into the labrum (pycnogonids lack a labrum). However, expression data of Hox genes that were published shortly afterwards suggested that the chelifores were deuterocerebral and thus most likely to be homologous to the chelicerae. The pycnogonids are thus neutral with regard to the great appendage theory.
Dieter Waloszek and colleagues have offered a rather different account of Cambrian arthropod head structure. They do not necessarily accept the primary antenna theory of the onychophoran antennae; and they reject the idea that Fuxianhuia or any of its close relatives possessed a great appendage. Rather, they place the "great appendage" arthropods in the stem-group of the chelicerates, arguing that the great appendage is homologous to the chelicerae of chelicerates, and the first antennae of crustaceans.
Trevor Cotton and Simon Braddy, in a comprehensive cladistic analysis of Cambrian arthropods, also proposed that the great appendage arthropods were stem-group chelicerates;and, accepting that Fuxianhuia and relatives possessed two preoral appendages, defended the classical view that the great appendage and the chelicerae were tritocerebral in origin; i.e. that the antennae of Fuxianhuia were deuterocerebral.
The number and nature of the post-oral segments in the insect head have rarely been questioned. A much more difficult area, however, has been the nature of the preoral region. The obvious contradiction between a theory that no-preoral structures are segmental, and evidence, such as for the first antennae of crustaceans, that some such structures clearly are, led workers as long ago as Lankester to posit that there has been forward migration of segments in front of the mouth. Indeed, such a process can be seen in ontogeny of the tritocerebrum, which can be seen to migrate forward as the brain develops; furthermore, although in most insects and crustaceans its ganglia are part of the brain, its commissures still loop behind it, suggesting derivation from a more posterior position.
Nevertheless, even allowing for this possibility, the complexity of the anterior part of the brain, which even if the acron concept is incorrect may still have been inherited from very basal animals; untangling the new characters evolved by the earliest arthropods from those inherited from their ancestors therefore still stands centrally in the arthropod head problem.