Zero Zero was published in a typical 6 1/2" x 9 3/4" comic book format. Issues ranged between 40 and 64 pages in length, printed mostly in black-and-white with a color cover but occasionally including sections printed in one or two colors, notably a series of stories by Al Columbia. Zero Zero's release schedule was irregular, fluctuating between bimonthly and quarterly intervals over the course of its run. A total of 27 issues were released.
The back cover of each issue of Zero Zero featured a captioned illustration depicting a numbered "Sign of the Impending Apocalypse", implying that the anthology's title is an oblique reference to the coming millennium. For the final issue this feature was replaced with an Al Columbia strip depicting the demise of an experimental subject named "Cheapy the Guinea Pig," which can be taken as a metaphor for the end of Zero Zero itself.