Tyndale House has typically been used by doctoral students from the University of Cambridge studying either in the Faculty of Divinity or the Faculty of Oriental Studies. Students from other universities, chiefly from the United Kingdom, but also from other places throughout the world, have done all or a portion of their work in residence there. In addition, numerous professors and independent scholars have taken a sabbatical or other time at Tyndale House to engage in a period of focused work. Scholars who have spent time at Tyndale House include: D.A. Carson, Wayne Grudem, J.I. Packer, and John Piper.
In 2007 Tyndale House launched the Kirby Laing Institute for Christian Ethics, known as KLICE.