The single-panel gag cartoon was a daily look at Toonerville, situated in what are now called the suburbs. Central to the strip was the rickety little trolley called the "Toonerville Trolley that met all the trains," driven in a frenzy by the grizzly old Skipper to meet each commuter train as it arrived in town. A few of the many richly-formed characters included The Terrible-Tempered Mr. Bang, The Powerful Katrinka, Little Woo-Woo Wortle, Aunt Eppie Hogg and Mickey McGuire, the town bully.
Between 1920 and 1922, 17 Toonerville silent film comedy adaptations were scripted by Fox for Philadelphia's Betzwood Film Company. These starred Dan Mason as the Skipper with Wilma Wild as Katrinka. Only seven of those 17 shorts survive today. Four are preserved in the Betzwood Film Archive at Montgomery County Community College in Blue Bell, Pennsylvania.
Mickey Rooney starred as Mickey McGuire in more than 55 comedy shorts filmed between 1927 and 1936. Rooney (né Joe Yule, Jr.) adopted the professional name Mickey McGuire for a time before finally settling on the last name Rooney. The first of several Van Beuren Studios animated cartoons adapted from the syndicated panels was released by RKO on January 17, 1936. Some of these became available on laserdisc in 1994 and later on DVD from Image Entertainment in 1999.
Over the years, various Toonerville characters acted as spokemen for popular products of the day. Skipper, Flem, and Katrinka appeared throughout the decades in advertisements for Drano, Kellogg's cereals, and Chef Boyardee foods.
Between 1934 and 1940, comic book reprints of the panel appeared in many issues of All-American Comics, Famous Funnies and Popular Comics. In 1995, the strip was one of 20 included in the Comic Strip Classics series of commemorative United States postage stamps.