The third ventricle (ventriculus tertius) is one of four connected fluid-filled cavities comprising the ventricular system within the human brain. It is a median cleft between the two thalami, and is filled with cerebrospinal fluid (CSF).
It is in the midline, between the left and right lateral ventricles.
It communicates with the fourth ventricle posteriorly by the cerebral aqueduct (of Sylvius).
In casts of the ventricular system, a small 'hole' may be seen in the body of the third ventricle. This is formed where the two thalami are joined together at the interthalamic adhesion (not seen in all people).