In ornithology:

• Black-headed Bunting, passerine bird in the bunting family Emberizidae
• Black-headed Cuckoo-shrike, species of cuckooshrike found in south and south-east Asia
• Black-headed Gull, small gull which breeds in much of Europe and Asia
• Black-headed Heron, wading bird of the heron family Ardeidae
• Black-headed Ibis, species of wading bird of the ibis family Threskiornithidae
• Black-headed Lapwing, large lapwing
• Black-headed Munia, small passerine bird
• Black-headed Saltator, seedeating bird in the cardinal family
• Great Black-headed Gull, large gull

In primatology:

• Black-headed Marmoset, marmoset species endemic to Brazil
• Black-headed Night Monkey, night monkey species from South America
• Black-headed Spider Monkey, type of New World monkey
• Black-headed Uakari, New World primate from the Pitheciidae family