Synthoids seem to be molded from a biomimetic polymer-like material which can be programmed to assume human form, duplicating clothing, voice, and behavioural characteristics of the original subject. (In this respect, the synthoid concept is comparable to the T-1000 from the 1991 science fiction film Terminator 2: Judgment Day, but predating said movie by six years.) Created for deception and infiltration operations, synthoid units can superficially pass for genuine human beings, exhibiting intelligence and autonomous personality, albeit with loyalties ever acting in Cobra's interests. All synthoids are equipped with a self-destruct mechanism which can be remotely triggered to dissolve their bodies into the formless gray goo of their initial constitution — perhaps suggesting a conceptual origin in literary trappings of nanotechnology.
Cobra first used the synthoids to replace several G.I. Joe military commanders, including Duke, in a plot to undermine the team’s fighting capability. With the synthoids posing as committee members, the faux commanders proceeded to issue budget cuts and imposed crippling supply restrictions, aiming to eventually disband the Joe Team. Although their resources were strained to desperation, the Joes eventually caught wind of Cobra’s plan and managed to deflate it, rescuing the real committee. As for the synthoids, the one impersonating Duke approached Cobra Commander and gave his identity; when the Commander impulsively decided to dissolve him to verify his identity, however, he initiated the function at far too high a setting, causing all of the synthoids to deactivate and exposing their presence in the U.S. military.
In the two-part episode "There’s No Place Like Springfield", Cobra employed synthoids en masse in a scheme to extract classified information from G.I. Joe team member Shipwreck, who had secret weapons knowledge imparted to him under hypnotic suggestion. Cobra manages to covertly capture Shipwreck, placing him under unknowing surveillance in a mock town peopled with synthoid duplicates of his acquaintances in the hope of coercing the secret information from him. This version was upgraded so that the mass of the synthoid could be reconstituted for repeated use. This was the last instance of synthoids appearing in the G.I. Joe cartoon.
The synthoid technology later resurfaced in another Sunbow-produced series, The Transformers. In the episode "Only Human", a man named Snake — ostensibly an aging Cobra Commander, leader of Cobra — misleads several Autobots to download their personalities from robot form into synthoid bodies.