Beginning in 1980, Trifkovic has been a radio broadcaster for BBC World Service and Voice of America and later a correspondent covering southeast Europe for U.S. News & World Report and the Washington Times during which time he was an editor for the Belgrade magazine Duga.
Trifkovic has been an adjunct professor at the University of St Thomas in Houston, TX (1996-1997), and in August 1997 he joined the faculty of the short-lived Rose Hill College in Aiken, SC. In 1994-95 he was an unofficial spokesman for the Bosnian Serb government. Trifkovic has published op-eds and commentaries in The Times of London, the San Francisco Chronicle, the American Conservative and The Philadelphia Inquirer, and been a commentator on numerous national and international TV and radio programs, including the Oliver North Show on (MSNBC), CNN , CNN International, SKY News , BBC Radio 4 , BBC World Service and CBC. He has contributed to Liberty, the newspaper of the Serbian National Defense Council of America. Trifkovic has worked as a political consultant to Aleksandar Karađorđević and Vojislav Koštunica, and as an adviser to Biljana Plavšić. In February 2000 he testified to the Canadian House of Commons on the situation in the Balkans. In July 2000 he took part in a Congressional briefing organized by Rep. Kucinich. Trifković has worked as a political consultant to Alexander, Crown Prince of Yugoslavia and Former Yugoslav President Vojislav Koštunica, as an adviser to Biljana Plavšić, and as representative of the Republika Srpska in London. In March 2003 he testified as an expert witness for the defense before the International Criminal Tribunal for the Former Yugoslavia.
In June 2006 he gave the keynote address at a symposium on the Holocaust in Yugoslavia, 1941-1945, at Yad Vashem Center in Jerusalem.
He has edited, written the Foreword, and contributed a chapter to two books:
Trifkovic's most influential work to date, The Sword of the Prophet, has been described as "exceptionally fluid argument against militant Islam... Powerful stuff powerfully presented" by Ray Olson in Booklist, and "easily the most important (not to mention courageous) book written in response to 9/11 that I've read" by Jeffrey Rubin, Editor of the Conservative Book Club. Writing in The Spectator, Frank Johnson called it "the sort of book which the professional anti-racists, and the British mosques, might try to ban before long." "The arbiters of official Islam will not tell us what Islam is, only what they want it to be. For the truth, we must turn to Dr. Serge Trifkovic, a European historian of broad learning, sound philosophy and keen political insight," says Brian Mitchell, Washington Bureau Chief of Investors Business Daily. "[Trifkovic] examines, among many illuminating topics, the powerful anti-Semitism that is constantly promoted in the Islamic press," wrote Paul Weyrich in The Washington Dispatch. In the opinion of Don Feder, "In his excellent new book… Serge Trifkovic documents how Muhammad, like a mafia don, put out contracts on those who dared to criticize his august personage. Trifkovic also describes Muhammad’s genocidal campaign against Arabia’s Jewish tribes." "Dr. Trifkovic understands Islam... The problem of historical ignorance in today’s English-speaking world, where claims about far- away lands and cultures are made on the basis of domestic multiculturalist assumptions, are hit right on the head by the author," wrote Taki in The American Conservative. , while National Review Book Service warns that Trifkovic exposes what "Muslims, multiculturalists, and the media hope you never find out about Islam." "Trifkovic gives us the unvarnished, 'politically incorrect' truth about Islam... and what we must do if we wish to survive," is the verdict of the Conservative Book Club According to a recent review, "Serge Trifkovic writes a calm and thoughtful tome... [H]e is arguing that a significant number of people subscribe to militant Islam, and that such militancy is a natural result of following the actual teachings of Muhammad and the Koran... If you want to wrestle with harsh truths rather than PC fiction, this book is a good place to start," concludes Steven Greenhut, The Orange County Register senior editorial writer. Paul Eidelberg the president of the Yamin Israel party has called him a "man of extraordinary intellectual courage" in a review of his book.
He was called a "noted Islamophobe" by neoconservative author Stephen Schwartz. In 2003 he was accused by Schwartz of being a supporter of Slobodan Milošević, though he was a signatory to a letter which protested Milošević in 1996 and has condemned him in numerous publications.