Rowena is located at , just over a mile north of the border with Iowa, about 5 miles east of Sioux Falls, and 5 miles west of the Minnesota border, at an elevation of 1,411 feet (430 meters). The ZIP Code is 57056.
According to an 1895 atlas, Rowena had a population of 126 people. According to the 1950 census, Rowena had a population of 70. The United States Census Bureau no longer tracks Rowena as a separate community.
Actress Mamie Van Doren was born in Rowena.