• Shot (filming), a part of a film between two cuts
• Shot glass, a liquor measure
• Lead shot, small balls of shot generally used as weights or as firearms projectiles
• Round shot, a solid projectile used in muzzle-loading cannon
• Shot (ice hockey) an attempt to score a goal or points in this and other sports
• Shot put, an event in track and field athletics
• Showt or Shoţ a city in Iran
• Gunshot injury, to receive such an injury
• Nuclear testing, a nuclear weapon test
• Cartridge (firearms), the discharge of a round of ammunition
• Injection (medicine) via a syringe and hyperdermic needle
• Silk, shot silk is a type of silk
• Chain (length), a shot of chain, usually 15 fathoms or 90 feet long

SHOT may stand for:

• Society for the History of Technology, a professional organization for historians of technology
• Serious Hazards of Transfusion, a "haemovigilance" organization for monitoring blood transfusion errors in the United Kingdom

In popular culture:

• Shot (song), a song by the Finnish rock band The Rasmus
• Shot (Gruntruck album)
• The Sisters of Mercy, Shot Rev. 2.0, a video album by this rock band