• R27 (airship), a British rigid airship of World War One
• R27 (New York City Subway car), a model of New York City Subway rolling stock manufactured from 1960 to 1961
• R27 (Western Cape), a Regional Route in South Africa
• R27: Very toxic in contact with skin, a risk phrase in chemistry
• BMW R27, a 1960s single-cylinder BMW motorcycle
• Renault R27, a racing car built by the Renault F1 team for use during the 2007 Formula One season

R-27 may refer to :

• R-27 (missile), a Soviet nuclear missile
• USS R-27 (SS-104), an R-class coastal and harbor defense submarine of the United States Navy
• Vympel R-27, a medium-range air-to-air missile developed by the Soviet Union