peptide, organic compound composed of amino acids linked together chemically by peptide bonds. The peptide bond always involves a single covalent link between the α-carboxyl (oxygen-bearing carbon) of one amino acid and the amino nitrogen of a second amino acid. In the formation of a peptide bond from two amino acids, a molecule of water is eliminated. Small peptides with fewer than about ten constituent amino acids are called oligopeptides, and peptides with more than ten amino acids are termed polypeptides. Compounds with molecular weights of more than 10,000 (50-100 amino acids) are usually termed proteins. Organisms commonly contain appreciable quantities of low-molecular-weight peptides some arising from proteins while others are synthesized directly. Certain of these molecules are unusual in that they incorporate amino acids not found in proteins such as amino acids of the d-configuration. Among the biological peptides are many with physiological or antibacterial activity, such as the peptide hormones oxytocin and vasopressin; adrenocorticotropic hormone (ACTH), secreted by the pituitary gland; and several cyclic peptides, in which the amino-acid sequence forms a ring structure rather than a straight chain, such as the antibiotics tyrocidin and gramicidin. Laboratory synthesis of peptides has risen to the level of a well-defined art in recent years. Synthetic peptides, composed of as many as a hundred amino acids in specified sequence, have been prepared in the laboratory with good purity and high yields.

Organic compound composed of a series of amino acids linked by peptide bonds (see covalent bond) between a carbon atom of one and a nitrogen atom of the next. Peptide chains longer than a few dozen amino acids are called proteins. Biosynthesis of peptides from a succession of amino acids carried by transfer RNA molecules takes place on ribosomes and is catalyzed and controlled by enzymes. Many hormones, antibiotics, and other compounds that participate in life processes are peptides.

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Peptides (from the Greek πεπτίδια, "small digestibles") are short polymers formed from the linking, in a defined order, of α-amino acids. The link between one amino acid residue and the next is known as an amide bond or a peptide bond.

Proteins are polypeptide molecules (or consist of multiple polypeptide subunits). The distinction is that peptides are short and polypeptides/proteins are long. There are several different conventions to determine these, all of which have caveats and nuances.

Conventions

One convention is that those peptide chains that are short enough to be made synthetically from the constituent amino acids are called peptides rather than proteins. However, with the advent of better synthetic techniques, peptides as long as hundreds of amino acids can be made, including full proteins like ubiquitin. Native chemical ligation has given access to even longer proteins, so this convention seems to be outdated.

Another convention places an informal dividing line at approximately 50 amino acids in length (some people claim shorter lengths). However, this definition is somewhat arbitrary. Long peptides, such as the amyloid beta peptide linked to Alzheimer's disease, can be considered proteins; and small proteins, such as insulin, can be considered peptides.

Peptide classes

Here are the major classes of peptides, according to how they are produced: Ribosomal peptides : Are synthesized by translation of mRNA. They are often subjected to proteolysis to generate the mature form. These function, typically in higher organisms, as hormones and signaling molecules. Some organisms produce peptides as antibiotics, such as microcins. Since they are translated, the amino acid residues involved are restricted to those utilized by the ribosome. However, these peptides frequently have posttranslational modifications, such as phosphorylation, hydroxylation, sulfonation, palmitylation, glycosylation and disulfide formation. In general, they are linear, although lariat structures have been observed. More exotic manipulations do occur, such as racemization of L-amino acids to D-amino acids in platypus venom. Nonribosomal peptides : These peptides are assembled by enzymes that are specific to each peptide, rather than by the ribosome. The most common non-ribosomal peptide is glutathione, which is a component of the antioxidant defenses of most aerobic organisms. Other nonribosomal peptides are most common in unicellular organisms, plants, and fungi and are synthesized by modular enzyme complexes called nonribosomal peptide synthetases. These complexes are often laid out in a similar fashion, and they can contain many different modules to perform a diverse set of chemical manipulations on the developing product. These peptides are often cyclic and can have highly-complex cyclic structures, although linear nonribosomal peptides are also common. Since the system is closely related to the machinery for building fatty acids and polyketides, hybrid compounds are often found. Oxazoles, thiazoles often indicate that the compound was synthesized in this fashion. Peptones

See also Tryptone

Are derived from animal milk or meat digested by proteolytic digestion. In addition to containing small peptides, the resulting spray-dried material includes fats, metals, salts, vitamins and many other biological compounds. Peptone is used in nutrient media for growing bacteria and fungi. Peptide Fragments : Refer to fragments of proteins that are used to identify or quantify the source protein. Often these are the products of enzymatic degradation performed in the laboratory on a controlled sample, but can also be forensic or paleontological samples which have been degraded by natural effects.

Peptides in molecular biology

Peptides have received prominence in molecular biology in recent times for several reasons. The first and most important is that peptides allow the creation of peptide antibodies in animals without the need to purify the protein of interest. This involves synthesizing antigenic peptides of sections of the protein of interest. These will then be used to make antibodies in a rabbit or mouse against the protein.

Another reason is that peptides have become instrumental in mass spectrometry, allowing the identification of proteins of interest based on peptide masses and sequence. In this case the peptides are most often generated by in-gel digestion after electrophoretic separation of the proteins.

Peptides have recently been used in the study of protein structure and function. For example, synthetic peptides can be used as probes to see where protein-peptide interactions occur.

Inhibitory peptides are also used in clinical research to examine the effects of peptides on the inhibition of cancer proteins and other diseases.

Well-known peptide families in humans

The peptide families in this section are all ribosomal peptides, usually with hormonal activity. All of these peptides are synthesized by cells as longer "propeptides" or "proproteins" and truncated prior to exiting the cell. They are released into the bloodstream where they perform their signalling functions.

The Tachykinin peptides

• Substance P
• Kassinin
• Neurokinin A
• Eledoisin
• Neurokinin B

Vasoactive intestinal peptides

• VIP (Vasoactive Intestinal Peptide; PHM27)
• PACAP Pituitary Adenylate Cyclase Activating Peptide
• Peptide PHI 27 (Peptide Histidine Isoleucine 27)
• GHRH 1-24 (Growth Hormone Releasing Hormone 1-24)
• Glucagon
• Secretin

Pancreatic polypeptide-related peptides

• NPY
• PYY (Peptide YY)
• APP (Avian Pancreatic Polypeptide)
• PPY Pancreatic PolYpeptide

Opioid peptides

• Proopiomelanocortin (POMC) peptides
• Enkephalin pentapeptides
• Prodynorphin peptides

Calcitonin peptides

• Calcitonin
• Amylin
• AGG01

Other peptides

• B-type Natriuretic Peptide (BNP) - produced in myocardium & useful in medical diagnosis

Notes on terminology

• A polypeptide is a single linear chain of amino acids.
• A protein is one or more polypeptides more than about 50 amino acids long.
• An oligopeptide or (simply) a peptide is a polypeptide less than 30-50 amino acids long.
• A dipeptide has two amino acids.
• A tripeptide has three amino acids.
• A pentapeptide has five amino acids.
• An ''octapeptide has eight amino acids. (e.g., angiotensin II.
• A nonapeptide has nine amino acids (e.g., oxytocin).
• A decapeptide has ten amino acids (e.g., gonadotropin-releasing hormone & angotensin I).
• A neuropeptide is a peptide that is active in association with neural tissue.
• A peptide hormone is a peptide that acts as a hormone.

See also

• Peptidomimetics (such as peptoids and β-peptides) to peptides, but with different properties.
• bis-peptide
• Peptide synthesis
• Translation
• Ribosome
• Argireline

References

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