The 3 substrates of this enzyme are nitrous oxide, acceptor, and H2O, whereas its two products are nitric oxide and reduced acceptor.
This enzyme belongs to the family of oxidoreductases, specifically those acting on other nitrogenous compounds as donors with other acceptors. The systematic name of this enzyme class is nitrous-oxide:acceptor oxidoreductase (NO-forming). Other names in common use include nitrogen oxide reductase, and nitrous-oxide:(acceptor) oxidoreductase (NO-forming). This enzyme participates in nitrogen metabolism.
As of late 2007, 8 structures have been solved for this class of enzymes, with PDB accession codes , , , , , , , and .