Madonna may refer to:

• Mary (mother of Jesus), from which other uses generally derive
• Madonna (art), a portrait of Mary
• Madonna and Child, portraits of Mary and the infant Christ
• Madonna (entertainer), American singer and actress
• Madonna (Madonna album), the entertainer's first self-titled album
• Madonna (video), a music video collection
• Madonna (Edvard Munch), a painting by Edvard Munch
• Madonna (...And You Will Know Us by the Trail of Dead album)
• Madonna, the top F1 team in the video games Super Monaco GP and Ayrton Senna's Super Monaco GP II

See also

• Madonna del Ghisallo, a hill close to Lake Como in Italy
• Madonna di Campiglio, a village and a ski resort in Trentino, Italy
• Madonna Inn, a resort hotel in California
• Madonna lily, a plant in the genus Lilium
• The Madonna of Port Lligat, two paintings by Salvador Dalí
• Madonna of the Trail, a series of historical monuments from Maryland to California
• Madonna Oriente, a moon goddess
• Madonna piercing, a type of facial piercing that can resemble a mole
• Madonna University, a college in Livonia, Michigan
• Black Madonna, a statue or painting of Mary in which she is depicted with black skin
• "Black Madonna", a song by Noe Venable
• House of the Black Madonna, a cubist building in Prague, Czech Republic
• "Lady Madonna", a song by The Beatles