Usually, a cell that is secreting the protein of interest is isolated using an antibody-antibody complex that coats the cell and is able to "catch" the secreted molecules. The cell is then detected by another fluorochrome-labelled antibody, and is subsequently extracted using a process called fluorescent-activated cell sorting (FACS). The FACS method is broadly similar to the enzyme-linked immunosorbent assay (ELISA) antibody format, except that the encapsulated cells remain intact. This is advantageous as the cells are still living after the extraction has taken place.
Further advances now mean that it is possible to extract the secreting cells using a magnetic-based separation system or using a flow cytometer.
A number of commercial applications exist for secretion assay. One such example is the Gel Microdrop (GMD) techonology, developed by One Cell Systems. One Cell asserts that GMD typically recovers a higher number of viable secreting cells than other methods, whilst ignoring any cells which are not secreting the desired protein.
Enzyme-Linked Immunosorbent Assay ELISA. - Global Strategic Business Report Provides Estimates for the Period of 2007 through to 2015.(Report)
Jul 20, 2010; Research and Markets (httpwww.researchandmarkets.com/research/565435/enzymelinked_immu) has announced the addition of the...
Development of 2 types of competitive enzyme-linked immunosorbent assay for detecting antibodies to the rinderpest virus using a monoclonal antibody for a specific region of the hemagglutinin protein.(Report)
Jun 01, 2007; Abstract: A competitive enzyme-linked immunosorbent assay (C-ELISA) has been developed and standardized for the detection of...