NP-40

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3.NP-40 is a commercially available detergent. The full name of NP-40 is Tergitol-type NP-40, which is nonyl phenoxylpolyethoxylethanol.

Care should be taken to avoid confusing NP-40 with Nonidet P-40 (octyl phenoxylpolyethoxylethanol). Nonidet P-40 is no longer sold by the chemical company, Sigma-Aldrich. Sigma-Aldrich has replaced Nonidet P-40 with Igepal CA-630, which is described as a "nonionic, non-denaturing detergent". Sigma-Aldrich claims that Igepal CA-630 is "chemically indistinguishable from Nonidet P-40".

This detergent is not powerful enough to break the nuclear membrane, but can break the cytoplasmic membrane. As such, it can be used to obtain the cytoplasmic contents of a cellular culture.

See also

• Surfactant
• Lipid bilayer