The 16 Heats began at 10:00, on March 25, 2007, at the Rod Laver Arena, in Melbourne Park.
The semifinals started on the evening of the same day at 20:03.
The Final started at 19:00 on the following day, March 26.
| WR | 59.13 | Brendan Hansen | Irvine | August 1, 2006 | |
| CR | 59.37 | Brendan Hansen | Montreal | July 25, 2005 |
| # | Heat | Lane | Name | Nationality | 50m | Time | Q |
|---|---|---|---|---|---|---|---|
| 1 | 14 | 5 | Kosuke Kitajima | 28.33 | 59.96 | Q | |
| 2 | 15 | 5 | Alexander Dale Oen | 28.59 | 1:00.34 | Q | |
| 3 | 16 | 4 | Brendan Hansen | 28.66 | 1:00.59 | Q | |
| 4 | 9 | 4 | Giergrius Titenis | 28.76 | 1:00.92 | Q | |
| 5 | 14 | 4 | Oleg Lisogor | 28.83 | 1:00.99 | Q | |
| 6 | 14 | 3 | James Gibson | 28.26 | 1:01.03 | Q | |
| 7 | 14 | 3 | Valeriy Dymo | 28.82 | 1:01.06 | Q | |
| 7 | 15 | 4 | Brenton Rickard | 28.77 | 1:01.06 | Q | |
| 9 | 16 | 3 | Hugues Dubscq | 28.85 | 1:01.08 | Q | |
| 10 | 12 | 2 | Cameron van der Burgh | 28.73 | 1:01.12 | Q | |
| 11 | 11 | 4 | Mihail Alexandrov | 28.60 | 1:01.15 | Q | |
| 12 | 16 | 2 | Scott Usher | 29.31 | 1:01.36 | Q | |
| 13 | 16 | 6 | Christopher Cook | 29.00 | 1:01.49 | Q | |
| 14 | 15 | 7 | Alessandro Terrin | 28.38 | 1:01.61 | Q | |
| 15 | 14 | 2 | Dmitry Komornikov | 28.95 | 1:01.66 | Q | |
| 16 | 15 | 8 | Robin van Aggele | 28.67 | 1:01.69 | ||
| 16 | 16 | 5 | Roman Sludnov | 29.09 | 1:01.69 | Q | |
| 18 | 16 | 1 | Mike Brown | 29.13 | 1:01.75 | ||
| 19 | 12 | 4 | Glenn Snyders | 29.36 | 1:01.91 | ||
| 20 | 11 | 3 | Jiri Jedlicka | 29.23 | 1:01.92 | ||
| 21 | 16 | 7 | Edoardo Giorgetti | 29.78 | 1:01.95 | ||
| 22 | 15 | 6 | Makoto Yamashita | 29.12 | 1:02.08 | ||
| 23 | 14 | 8 | Henrique Barbosa | 28.81 | 1:02.13 | ||
| 24 | 13 | 1 | Lai Zhongjian | 29.62 | 1:02.18 | ||
| 25 | 15 | 1 | Valentin Preda | 28.74 | 1:02.19 | ||
| 26 | 15 | 2 | Richard Bodor | 29.20 | 1:02.28 | ||
| 27 | 13 | 2 | Jim Piper | 29.06 | 1:02.36 | ||
| 28 | 14 | 1 | Johannes Neumann | 29.11 | 1:02.50 | ||
| 29 | 13 | 8 | Slawomir Wolniak | 29.06 | 1:02.50 | ||
| 30 | 14 | 7 | Felipe Lima | 29.23 | 1:02.58 | ||
| 31 | 12 | 5 | Maxim Podoprigora | 29.76 | 1:02.71 | ||
| 31 | 12 | 8 | Thabang Moeketsane | 29.05 | 1:02.71 | ||
| 33 | 11 | 5 | Mladen Tepavcevic | 29.42 | 1:02.80 | ||
| 33 | 13 | 4 | Slawomir Kuczko | 29.81 | 1:02.80 | ||
| 35 | 12 | 1 | Chris Boe Christensen | 29.84 | 1:02.83 | ||
| 36 | 16 | 8 | Emil Tahirovic | 28.52 | 1:02.85 | ||
| 37 | 13 | 6 | Jakob Johann Sveinsson | 29.47 | 1:02.91 | ||
| 38 | 11 | 6 | Edvinas Dautartas | 29.07 | 1:03.07 | ||
| 39 | 10 | 6 | Sofiane Diad | 29.31 | 1:03.09 | ||
| 40 | 12 | 6 | Daniel Gyurta | 30.13 | 1:03.33 | ||
| 41 | 13 | 5 | Wang Haibo | 29.99 | 1:03.38 | ||
| 42 | 12 | 7 | Malick Fall | 29.62 | 1:03.42 | ||
| 43 | 10 | 7 | Tomas Fucik | 29.59 | 1:03.44 | ||
| 44 | 15 | 3 | Romanos Iasonas Alyfantis | 29.40 | 1:03.51 | ||
| 45 | 11 | 2 | Mikalai Vasilyeu | 29.61 | 1:03.62 | ||
| 46 | 12 | 3 | Yevgeniy Ryzhkov | 29.99 | 1:03.70 | ||
| 47 | 10 | 2 | Tom Beeri | 30.07 | 1:03.72 | ||
| 47 | 10 | 1 | Michael Malul | 29.65 | 1:03.72 | ||
| 49 | 11 | 8 | Alwin de Prins | 29.69 | 1:04.00 | ||
| 50 | 11 | 7 | Martti Aljand | 30.31 | 1:04.13 | ||
| 51 | 13 | 7 | Matjaz Markic | 28.96 | 1:04.20 | ||
| 52 | 9 | 3 | Sujong Sin | 30.46 | 1:04.31 | ||
| 53 | 10 | 3 | Hsin Hung Chiang | 30.25 | 1:04.49 | ||
| 54 | 9 | 6 | Dean Kent | 30.20 | 1:04.51 | ||
| 55 | 10 | 4 | Nikola Delic | 29.68 | 1:04.58 | ||
| 56 | 9 | 5 | Andrei Capitanciuc | 30.24 | 1:04.76 | ||
| 57 | 10 | 8 | Dimitri Waeber | 29.88 | 1:04.80 | ||
| 58 | 7 | 6 | Wael Kobrosly | 30.66 | 1:04.83 | ||
| 59 | 8 | 1 | Vidvuds Maculevics | 30.17 | 1:05.36 | ||
| 60 | 11 | 1 | Wei Wen Wang | 30.79 | 1:05.38 | ||
| 61 | 9 | 7 | Sergiu Postica | 30.24 | 1:05.45 | ||
| 62 | 9 | 2 | Mohammad Alirezaei Dizicheh | 29.99 | 1:05.64 | ||
| 63 | 7 | 3 | Radomyos Matjiur | 30.65 | 1:05.70 | ||
| 64 | 8 | 2 | Diego Bonilla | 30.79 | 1:05.75 | ||
| 65 | 9 | 8 | Herry Yudhianto | 31.39 | 1:05.81 | ||
| 66 | 9 | 1 | Andrei Cross | 29.91 | 1:05.91 | ||
| 67 | 8 | 4 | Edgar Robert Crespo | 31.08 | 1:06.00 | ||
| 68 | 7 | 4 | Rainui Terupaia | 30.37 | 1:06.10 | ||
| 69 | 8 | 5 | Jin Leonard Tan | 30.75 | 1:06.14 | ||
| 70 | 8 | 6 | Andrey Morkovin | 31.36 | 1:06.46 | ||
| 70 | 8 | 7 | Billy Arfianto | 31.70 | 1:06.46 | ||
| 72 | 10 | 5 | Kirils Sanzarovecs | 30.54 | 1:06.65 | ||
| 73 | 7 | 1 | Kevin Hensley | 30.56 | 1:06.86 | ||
| 74 | 6 | 7 | Josh Laban | 31.66 | 1:07.28 | ||
| 75 | 8 | 3 | Gerard Bordado | 31.12 | 1:07.37 | ||
| 76 | 8 | 8 | Kee Guan Benjamin Ng | 31.36 | 1:07.92 | ||
| 77 | 6 | 5 | Milan Glamocic | 31.50 | 1:07.99 | ||
| 78 | 7 | 7 | Yusuf Alyousuf | 31.97 | 1:08.22 | ||
| 79 | 7 | 2 | Mustafa Al-Saleh | 32.17 | 1:08.34 | ||
| 80 | 7 | 8 | See Tuan Yap | 31.78 | 1:08.39 | ||
| 81 | 6 | 8 | Eric Williams | 31.94 | 1:08.77 | ||
| 82 | 7 | 5 | Genaro Mathias Prono Britez | 31.56 | 1:08.89 | ||
| 83 | 6 | 6 | Erik Rajohnson | 32.70 | 1:09.01' | ||
| 84 | 6 | 4 | Antonio Leon Candia | 31.11 | 1:09.32 | ||
| 85 | 5 | 4 | Amar Shah | 31.53 | 1:09.67 | ||
| 86 | 4 | 5 | Celestino Aguon | 32.88 | 1:10.46 | ||
| 87 | 6 | 2 | Chisela Kanchela | 32.01 | 1:10.64 | ||
| 88 | 5 | 3 | Melvin Chua | 33.04 | 1:10.65 | ||
| 89 | 4 | 4 | Hei Ming Lao | 33.01 | 1:10.84 | ||
| 90 | 3 | 4 | Boldbaataryn Bütekh-Uils | 32.50 | 1:11.06 | ||
| 91 | 5 | 1 | Rodion Davelaar | 33.59 | 1:11.38 | ||
| 92 | 4 | 7 | Omar Jasim | 32.38 | 1:11.38 | ||
| 93 | 5 | 5 | Alexander Rivero | 32.49 | 1:11.62 | ||
| 94 | 6 | 1 | Osama Al Araj | 32.92 | 1:11.73 | ||
| 95 | 5 | 7 | Aram Nazaryan | 33.18 | 1:12.21 | ||
| 96 | 5 | 8 | Jehad Al Henidi | 33.13 | 1:12.72 | ||
| 97 | 4 | 8 | Guvanch Ataniyazov | 33.67 | 1:13.12 | ||
| 98 | 4 | 1 | Eric Artur Medina Su | 33.86 | 1:13.17 | ||
| 99 | 5 | 2 | Onan Thom | 34.20 | 1:13.62 | ||
| 100 | 4 | 6 | Kevin Cheung Ho Yan | 34.05 | 1:13.87 | ||
| 101 | 4 | 2 | Gavin Sants | 34.69 | 1:13.88 | ||
| 102 | 5 | 6 | Jamie Zammitt | 33.91 | 1:14.43 | ||
| 103 | 2 | 3 | A. Aldhaheri | 35.72 | 1:15.93 | ||
| 104 | 3 | 6 | Eli Ebenezer Wong | 35.72 | 1:15.3 | ||
| 105 | 3 | 2 | Julio Smith | 34.48 | 1:16.55 | ||
| 106 | 3 | 3 | Sadeq Damrah | 34.92 | 1:16.60 | ||
| 107 | 3 | 1 | Hassan Ashraf | 35.15 | 1:17.26 | ||
| 108 | 3 | 8 | Michael Taylor | 36.22 | 1:17.79 | ||
| 109 | 3 | 5 | Monder Al-Jabali | 35.89 | 1:20.40 | ||
| 110 | 2 | 6 | Rahim Karmali | 37.32 | 1:21.27 | ||
| 111 | 1 | 5 | Jackson Niyomugabo | 39.45 | 1:25.87 | ||
| 112 | 2 | 8 | Anthony Kpetnky | 33.86 | 1:13.7 | ||
| 113 | 1 | 3 | Seele Benjamin Ntai | 42.54 | 1:31.58 | ||
| 114 | 1 | 4 | Boipelo Makhothi | 42.16 | 1:32.63 | ||
| 115 | 2 | 1 | Vincent Leatualevao Jr. | 41.39 | 1:32.86 | ||
| 116 | 2 | 5 | Robert Scanlan | 41.55 | 1:34.96 | ||
| 117 | 2 | 4 | Abubakarr Jalloh | 45.22 | 1:44.93 | ||
| ' | 2 | 2 | M. Alhousseini Alhassan | DNS | |||
| ' | 2 | 7 | Julio Yao Bessan | DNS | |||
| ' | 3 | 7 | Thi Hue Pham | DNS | |||
| ' | 4 | 3 | João Aguiar | DNS | |||
| ' | 6 | 3 | Shajahan Ali Rony | DNS | |||
| ' | 13 | 3 | Vanja Rogulj | DNS | |||
| # | Heat | Lane | Name | Nationality | 50m | Time | Q |
|---|---|---|---|---|---|---|---|
| 1 | 2 | 4 | Kosuke Kitajima | 28.31 | 1:00.05 | Q | |
| 2 | 2 | 5 | Brendan Hansen | 28.09 | 1:00.13 | Q | |
| 3 | 1 | 4 | Alexander Dale Ole | 28.52 | 1:00.69 | Q | |
| 4 | 1 | 6 | Brenton Rickard | 28.51 | 1:00.87 | Q | |
| 5 | 2 | 6 | Valeriy Dymo | 28.64 | 1:00.88 | Q | |
| 6 | 2 | 3 | Oleg Lisogor | 28.86 | 1:00.89 | Q | |
| 7 | 2 | 7 | Mihail Alexandrov | 28.33 | 1:01.08 | Q | |
| 8 | 2 | 8 | Dmitry Komornikov | 28.87 | 1:01.11 | Q | |
| 9 | 1 | 3 | James Gibson | 27.95 | 1:01.24 | ||
| 9 | 1 | 7 | Scott Usher | 29.01 | 1:01.24 | ||
| 11 | 2 | 1 | Christopher Cook | 28.15 | 1:01.28 | ||
| 12 | 1 | 5 | Giedrius Titenis | 28.82 | 1:01.48 | ||
| 13 | 1 | 8 | Roman Sludnov | 28.69 | 1:01.50 | ||
| 14 | 1 | 2 | Cameron van der Burgh | 28.76 | 1:01.80 | ||
| 15 | 1 | 1 | Alessandro Terrin | 28.33 | 1:01.87 | ||
| 16 | 2 | 2 | Hugues Duboscq | 28.80 | 1:01.93 | ||
| # | Lane | Name | Nationality | 50m | Time |
|---|---|---|---|---|---|
| 5 | Brendan Hansen | 27.67 | 59.80 | ||
| 4 | Kosuke Kitajima | 27.79 | 59.96 | ||
| 6 | Brenton Rickard | 28.15 | 1:00.58 | ||
| 4 | 2 | Valeriy Dymo | 28.18 | 1:00.60 | |
| 5 | 7 | Oleg Lisogor | 28.07 | 1:00.83 | |
| 6 | 1 | Mihail Alexandrov | 28.09 | 1:01.17 | |
| 7 | 8 | Dmitry Komornikov | 28.58 | 1:01.24 | |
| 8 | 3 | Alexander Dale Oen | 27.93 | 1:01.67 | |