Rasputin first appeared summoning the Beast of the Apocalypse for the Nazis during World War II. When the summoning accidentally placed the creature (Hellboy) in the hands of the Allies, Rasputin retreated to the Arctic Circle.
He returned in 1994 with Sadu-Hem, a monstrous spawn of the Ogdru Jahad, in tow. Rasputin's goal was to use Hellboy's stone right hand to free the Ogdru Jahad from their crystal prisons and permit them to raze the earth. However, Rasputin's plans were foiled by the timely intervention of Hellboy's associates from the Bureau for Paranormal Research and Defense, Liz Sherman and Abe Sapien. Rasputin was killed and Sadu-Hem was burned to death.
Rasputin re-appeared as a ghost while his pawns attempted to restore the vampire Vladimir Giurescu to life. Rasputin directed Ilsa Haupstein, a loyal member of Project Ragna Rok, to abandon her efforts to revive Giurescu and instead place herself within an enchanted iron maiden. Haupstein did so, dying in the process, but her blood allowed the goddess Hecate to use the iron maiden as a permanent and indestructible body. Hecate's mortal form, like that of Rasputin himself, had been destroyed.
Rasputin appeared lastly in his ghostly form as a counsel to Inger von Klempt. He persuaded her that she was to be the first of a final race of men, but she was executed by Lobster Johnson and the end of the world was averted with the destruction of the Conqueror Worm. Rasputin retreated to the roots of the World Tree, where the Baba Yaga took what was left of his soul and placed it in an acorn.
When the BPRD investigated the disappearance of a regenerated Sadu Hem from a Bureau facility in New Jersey, they found a doomsday cult led by an acolyte of Rasputin. The acolyte, who had exhibited a chest wound the day that Rasputin was speared to death, fulfilled Rasputin's prophecy regarding his murderer. Abe Sapien, the agent that slew Rasputin, was himself speared to death by the acolyte. Sapien, however, was soon revived and both the acolyte and Sadu Hem were killed.
Rasputin's appearance in this story was limited to a photograph in a shrine.
On Hellboy II: The Golden Army, director Guillermo Del Toro intented to show a after-credits scene of Rasputin's ghost aiding Roderick Zinco to ressurect Kroenen. That was meant to be a follow-up to the third movie, but was cut off for budget reasons.