The atomic mass unit (amu) is an older name for the same thing (unified atomic mass unit, dalton, or universal mass unit).
In biochemistry and molecular biology, when talking about proteins, the term "kiloDalton" is used, with the symbol kDa. Because proteins are large molecules, their masses are in kiloDaltons, where one kiloDalton is 1000 daltons.
The unified atomic mass unit, or Dalton, is not an SI unit of mass, but it is accepted for use with SI under either name.
The unit is convenient because one hydrogen atom has a mass of approximately 1 u, and more generally an atom or molecule that contains n protons and neutrons will have a mass approximately equal to n u. (The reason is that a atom contains 6 protons, 6 neutrons and 6 electrons, with the protons and neutrons having about the same mass and the electron mass being negligible in comparison.The mass of the electron is approximately 1/1836 of the mass of the proton.) This is an approximation, since it does not account for the mass contained in the binding energy of an atom's nucleus; this binding energy mass is not a fixed fraction of an atom's total mass. The differences which result from nuclear binding are generally less than , however. Chemical element masses, as expressed in u, would therefore all be close to whole number values (within 2% and usually within 1%) were it not for the fact that atomic weights of chemical elements are averaged values of the various stable isotope masses in the abundances which they naturally occur. For example, chlorine has an atomic weight of because it is composed of 76% and 24% ().
Another reason the unit is used is that it is experimentally much easier and more precise to compare masses of atoms and molecules (determine relative masses) than to measure their absolute masses. Masses are compared with a mass spectrometer (see below).
Avogadro's number (NA) and the mole are defined so that one mole of a substance with atomic or molecular mass will have a mass of precisely . For example, the molecular mass of a water molecule containing one isotope and two isotopes is , and this means that one mole of this monoisotopic water has a mass of . Water and most molecules consist of a mixture of molecular masses due to naturally occurring isotopes. For this reason these sort of comparisons are more meaningful and practical using molar masses which are generally expressed in g/mol, not u. In other words the one-to-one relationship between daltons and g/mol is true but in order to be used accurately for any practical purpose any calculations must be with isotopically pure substances or involve much more complicated statistical averaging of multiple isotopic compositions.
Before 1961, the physical atomic mass unit (amu) was defined as of the mass of one atom of oxygen-16, while the chemical atomic mass unit (amu) was defined as of the average mass of an oxygen atom (taking the natural abundance of the different oxygen isotopes into account). Both units are slightly smaller than the unified atomic mass unit, which was adopted by the International Union of Pure and Applied Physics in 1960 and by the International Union of Pure and Applied Chemistry in 1961. Hence, before 1961 physicists as well as chemists used the symbol amu for their respective (and slightly different) atomic mass units. One still sometimes finds this usage in the scientific literature today. However, the accepted standard is now the unified atomic mass unit (symbol u), with: 1 u = 1.000 317 9 amu (physical scale) = 1.000 043 amu (chemical scale). Since 1961, by definition the unified atomic mass unit is equal to one-twelfth of the mass of a carbon-12 atom.