, a glutathionylspermidine synthase
is an enzyme
the chemical reaction
- glutathione + spermidine + ATP glutathionylspermidine + ADP + phosphate
The 3 substrates of this enzyme are glutathione, spermidine, and ATP, whereas its 3 products are glutathionylspermidine, ADP, and phosphate.
This enzyme belongs to the family of ligases, specifically those forming carbon-nitrogen bonds as acid-D-ammonia (or amine) ligases (amide synthases). The systematic name of this enzyme class is gamma-L-glutamyl-L-cysteinyl-glycine:spermidine ligase (ADP-forming) [spermidine is numbered so that atom N-1 is in the amino group of the aminopropyl part of the molecule]. This enzyme is also called glutathione:spermidine ligase (ADP-forming). This enzyme participates in glutathione metabolism. It employs one cofactor, magnesium.
As of late 2007, 5 structures have been solved for this class of enzymes, with PDB accession codes , , , , and .
- Smith K, Nadeau K, Bradley M, Walsh C, Fairlamb AH "Purification of glutathionylspermidine and trypanothione synthetases from Crithidia fasciculata". Protein. Sci. 1 874–83.
- Bollinger JM Jr, Kwon DS, Huisman GW, Kolter R, Walsh CT "Glutathionylspermidine metabolism in Escherichia coli. Purification, cloning, overproduction, and characterization of a bifunctional glutathionylspermidine synthetase/amidase". J. Biol. Chem. 270 14031–41.
- The CAS registry number for this enzyme class is .
Gene Ontology (GO) codes