In 1832, he participated in the foundation of the Société entomologique de France. The following year he became an aide-naturaliste (assistant naturalist) to Jean Victoire Audouin in charge of Crustacea, Arachnida and insects.
Brullé studied for and obtained a baccalauréat in sciences then in "lettres", before qualifying in 1839 as a Doctor of Natural Science. His thesis, published in 1837, was Sur le gisement des insectes fossiles et sur les services que l'étude de ces animaux peut fournir à la géologie. He became the Professor of Zoology and Comparative Anatomy at the University of Dijon.
He proposed a new classification of Neuroptera which was completed by Wilhelm Ferdinand Erichson. He also wrote the introduction and parts of the text of Histoire naturelle des insectes coléoptères (published in 1840) with Francis de Laporte de Castelnau.