Isoflavone 7-O-methyltransferase

S-adenosyl-L-methionine + a 7-hydroxyisoflavone $rightleftharpoons$ S-adenosyl-L-homocysteine + a 7-methoxyisoflavone

Thus, the two substrates of this enzyme are S-adenosyl methionine and 7-hydroxyisoflavone, whereas its two products are S-adenosylhomocysteine and 7-methoxyisoflavone.

This enzyme belongs to the family of transferases, specifically those transferring one-carbon group methyltransferases. The systematic name of this enzyme class is S-adenosyl-L-methionine:hydroxyisoflavone 7-O-methyltransferase. This enzyme participates in isoflavonoid biosynthesis.

References

• Edwards R and Dixon RA "Isoflavone O-methyltransferase activities in elicitor-treated cell suspension cultures of Medicago sativa". Phytochemistry 30 2597–2606.
• He XZ, Dixon RA "Genetic manipulation of isoflavone 7-O-methyltransferase enhances biosynthesis of 4'-O-methylated isoflavonoid phytoalexins and disease resistance in alfalfa". Plant. Cell. 12 1689–702.
• He XZ, Dixon RA "Affinity chromatography, substrate/product specificity, and amino acid sequence analysis of an isoflavone O-methyltransferase from alfalfa (Medicago sativa L.)". Arch. Biochem. Biophys. 336 121–9.
• He XZ, Reddy JT, Dixon RA "Stress responses in alfalfa (Medicago sativa L). XXII. cDNA cloning and characterization of an elicitor-inducible isoflavone 7-O-methyltransferase". Plant. Mol. Biol. 36 43–54.
• Liu CJ, Dixon RA "Elicitor-induced association of isoflavone O-methyltransferase with endomembranes prevents the formation and 7-O-methylation of daidzein during isoflavonoid phytoalexin biosynthesis". Plant. Cell. 13 2643–58.
• Zubieta C, He XZ, Dixon RA, Noel JP "Structures of two natural product methyltransferases reveal the basis for substrate specificity in plant O-methyltransferases". Nat. Struct. Biol. 8 271–9.
• Christensen AB, Gregersen PL, Olsen CE, Collinge DB "A flavonoid 7-O-methyltransferase is expressed in barley leaves in response to pathogen attack". Plant. Mol. Biol. 36 219–27.

External links

The CAS registry number for this enzyme class is .