The Drosophilinae are the largest subfamily in the Drosophilidae. The other subfamily is the Steganinae.

Phylogeny

Many molecular studies have addressed small parts of the phylogenetic tree. Most of these studies are limited to species of the genus Drosophila. The genus Drosophila is paraphyletic as several genera, such as Zaprionus, Scaptomyza and Lordiphosa, are positioned within the genus. Position of the bolded species in the phylogenetic tree is at least reasonably well supported by existing molecular evidence.

Tribe: Drosophilini

Subtribe: Colocasiomyina

Genus: Baeodrosophila

Genus: Colocasiomyia

Genus: Palmomyia

Genus: Palmophila

Subtribe: Drosophilina

Infratribe: Drosophiliti

Genus: Bialba

Genus: Calodrosophila

Genus: Celidosoma

Genus: Chymomyza

Genus: Dettopsomyia

Genus: Dicladochaeta

Genus: Hypselothyrea

Genus: Jeannelopsis

Genus: Lissocephala

Genus: Marquesia

Genus: Microdrosophila

Genus: Mulgravea

Genus: Neotanygastrella

Genus: Paraliodrosophila

Genus: Poliocephala

Genus: Protochymomyza

Genus: Scaptodrosophila

Genus: Sphaerogastrella

Genus: Styloptera

Genus: Tambourella

Genus: Zaropunis

Genus: Drosophila including the following genera:

*Genus: Dichaetophora

*Genus: Hirtodrosophila

*Genus: Liodrosophila

*Genus: Lordiphosa

*Genus: Mycodrosophila

*Genus: Paramycodrosophila

*Genus: Phorticella

*Genus: Samoaia

*Genus: Scaptomyza

*Genus: Zaprionus

*Genus: Zygothrica

Infratribe: Laccodrosophiliti

Genus: Zapriothrica

Genus: Laccodrosophila

Genus: Cladochaeta

Genus: Diathoneura

Tribe unknown:

Genus: Miomyia

Genus: Collessia

Genus: Balara

For species within the various genera, see Taxodros