See biographies by J. P. Marquand (1925 and 1960).
Timothy is mentioned in the Bible at the time of St. Paul's second visit to Lystra (Acts 16:1-2), where Timothy is mentioned as a "disciple" or "follower." St. Paul, having been impressed by his "own son in the faith," arranged that he should become his companion (Acts 16:3), and personally circumcised him because his mother was of the Jewish faith, so that he might be accepted by the Jews. He was ordained (1 Timothy 4:14) and went with Paul in his journey through Phrygia, Galatia and Mysia; also to Troas, Philippi, Berea (Acts 17) and Corinth (Acts 18:5). His mother, Eunice, and his grandmother, Lois, are noted as eminent for their piety and faith, which indicates that they may have also been Christians. Timothy is praised by Paul for his knowledge of the Scriptures, and is said to have been acquainted with the Scriptures since childhood. The Bible gives little information about Timothy's father; however, it does indicate that he was a Greek (Acts 16:1).
According to later tradition, St. Paul ordained St. Timothy as Bishop of Ephesus in the year 65, where he served for 15 years. In the year 80 (though some sources place the event during the year 97, with Timothy dying at age 80), Timothy tried to halt a pagan procession of idols, ceremonies and songs. In response to his preaching of the Gospel, the angry pagans beat him, dragged him through the streets and stoned him to death. In the 4th century, his relics were transferred to the Church of the Holy Apostles in Constantinople.