A genetically modified organism (GMO) or genetically engineered organism (GEO) is an organism whose genetic material has been altered using genetic engineering techniques.These techniques are generally known as recombinant DNA technology. With this technology, DNA molecules from different sources are combined into one molecule to create a new set of genes. This DNA is then transferred into an organism, giving it modified or novel traits.
The general principle and foundation of producing a GMO is to add new genetic material into an organism's genome to generate new traits. This is called genetic engineering and was made possible through a series of scientific advances including the discovery of DNA and the creation of the first recombinant bacteria in 1973, i.e., E .coli expressing a salmonella gene. This led to concerns in the scientific community about potential risks from genetic engineering which have been thoroughly discussed at the Asilomar Conference in Pacific Grove, California. The recommendations laid out from this meeting were that government oversight of recombinant DNA research should be established until the technology was deemed safe. Herbert Boyer then founded the first company to use recombinant DNA technology, Genentech, and in 1978 the company announced the creation of an E. coli strain producing the human protein insulin.
In 1986, field tests of bacteria genetically engineered to protect plants from frost damage (ice-minus bacteria) at a small biotechnology company called Advanced Genetic Sciences of Oakland, California, were repeatedly delayed by opponents of biotechnology. In the same year, a proposed field test of a microbe genetically engineered for a pest resistance protein by Monsanto was dropped.
Genetically modified bacteria are used to produce the protein insulin, to treat diabetes. Similar bacteria have been used to produce clotting factors to treat haemophilia, and human growth hormone to treat various forms of dwarfism. These recombinant proteins are much safer than the products they replaced, since the older products were purified from cadavers and could transmit diseases. Indeed the human-derived proteins caused many cases of AIDS and hepatitis C in haemophilliacs and Creutzfeldt-Jakob disease from human growth hormone.
In addition to bacteria being used for producing proteins, genetically modified viruses allow gene therapy. Gene therapy is a relatively new idea in medicine. A virus reproduces by injecting its own genetic material into an existing cell. That cell then follows the instructions in this genetic material and produces more viruses. In medicine this process is adapted to deliver a gene that could cure disease into human cells. Although gene therapy is still relatively new, it has had some successes. It has been used to treat genetic disorders such as severe combined immunodeficiency, and treatments are being developed for a range of other incurable diseases, such as cystic fibrosis, sickle cell anemia, and muscular dystrophy.
For instance, the bacteria in your mouth which causes tooth decay is called Streptococcus mutans. This bacteria eats left over sugars in your mouth and produces acid that eats away tooth enamel and causes cavities. Scientists have recently modified Streptococcus mutans to produce ethanol. This transgenic bacterium, if properly colonized in a person's mouth, could possibly eliminate cavities and other tooth related issues. Transgenic microbes have also been used in recent research to kill or hinder tumors, and fight Crohn's disease. Genetically modified bacteria is also used in some soils to facilitate crop growth, and can also produce chemicals which are toxic to crop pests.
Transgenic animals are used as experimental models to perform phenotypic tests with genes whose function is unknown or to generate animals that are susceptible to certain compounds or stresses for testing in biomedical research. Other applications include the production of human hormones, such as insulin.
Frequently used in genetic research are transgenic fruit flies (Drosophila melanogaster) as genetic models to study the effects of genetic changes on development. Flies are often preferred over other animals for ease of culture, and also because the fly genome is somewhat simpler than that of vertebrates. Transgenic mice are often used to study cellular and tissue-specific responses to disease.
Transgenic plants have been developed for various purposes. Among many, these include 1) resistance to pests, herbicides or harsh environmental conditions, 2) improved product shelflife, and 3) increased nutritional value. Since the first commercial cultivation of GM plants in 1996, GM plants tolerant to the herbicides glufosinate or glyphosate, and producing the Bt toxin, an insecticide, have dominated the agriculutral seed market for corn and other crops. Recently, a new generation of GM plants promising benefits for consumers and industry purposes is entering the market.
Whenever GM plants are grown on open fields without containment there are risks that the modification will escape into the general environment. Most countries require biosafety studies prior to the approval of a new GM plant release, usually followed by a monitoring program to detect environmental impacts.
Especially in Europe, the coexistence of GM plants with conventional and organic crops has raised many concerns. Since there is separate legislation for GM crops and a high demand from consumers for the freedom of choice between GM and non-GM foods, measures are required to separate foods and feed produced from GMO plants from conventional and organic foods. European research programmes such as Co-Extra, Transcontainer and SIGMEA are investigating appropriate tools and rules. At the field level, these are biological containment methods, isolation distances and pollen barriers.
While some groups advocate the complete prohibition of GMOs, others call for mandatory labeling of genetically modified food or other products. Other controversies include the definition of patent and property pertaining to products of genetic engineering and the possibility of unforeseen local and global effects as a result of transgenic organisms proliferating. The basic ethical issues involved in genetic research are discussed in the article on genetic engineering.
Currently, there is little international consensus regarding the acceptability and effective role of modified "complete" organisms such as plants or animals. A great deal of the modern research that is illuminating complex biochemical processes and disease mechanisms makes vast use of genetic engineering.
The European Union funds research programmes such as Co-Extra, that investigate options and technologies on the coexistence of GM and conventional farming. This also includes research on biological containment strategies and other measures, that prevent outcrossing and enable the implementation of coexistence.
If patented genes are outcrossed, even accidentally, to other commercial fields and a person deliberately selects the outcrossed plants for subsequent planting then the patent holder has the right to control the use of those crops. This was supported in Canadian law in the case of Monsanto Canada Inc. v. Schmeiser.
In addition to the commercial protection of proprietary technology in self-pollinating crops such as soybean (a generally contentious issue) another purpose of the terminator gene is to prevent the escape of genetically modified traits from crosspollinating crops into wild-type species by sterilizing any resultant hybrids. The terminator gene technology created a backlash amongst those who felt the technology would prevent re-use of seed by farmers growing such terminator varieties in the developing world and was ostensibly a means to exercise patent claims. Use of the terminator technology would also prevent "volunteers", or crops that grow from unharvested seed, a major concern that arose during the Starlink debacle. There are technologies evolving which contain the transgene by biological means and still can provide fertile seeds using fertility restorer functions. Such methods are being developed by several EU research programmes, among them Transcontainer and Co-Extra.
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