Chelex_100
Chelex 100
Chelex 100 is a chelating resin used to purify other compounds via ion exchange. It is noteworthy for its ability to bind transition metal ions.
It is a styrene-divinylbenzene resin containing iminodiacetic acid groups.
A concentrated solution of metals is obtained by eluting the resin with a small volume of 2 M nitric acid, which protonates the iminodiacetate groups.
Chelex resin is often used for DNA extraction. Polar resin beads bind polar cellular components after breaking open cells. Non-polar nuclear DNA and RNA remain in water solution above chelex.
References
- Daniel Harris. Quantitative Chemical Analysis, seventh edition, 2007. ISBN: 0-7167-7041-5. Page 594.
- R. N. Ceo, M. R. Kazerouni, and K. Rengan. "Sorption of silver ions by Chelex 100 chelating resin." Journal of Radionanalytical and Nuclear Chemistry, 1992. Abstract.
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Last updated on Wednesday July 23, 2008 at 05:31:50 PDT (GMT -0700)
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This article is licensed under the GNU Free Documentation License.
Last updated on Wednesday July 23, 2008 at 05:31:50 PDT (GMT -0700)
View this article at Wikipedia.org - Edit this article at Wikipedia.org - Donate to the Wikimedia Foundation
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